Direct cytotoxicity against chicken erythrocytes in mice. I. Fundamental nature of T cell-mediated cytotoxicity

Chiharu Kubo, K. Nomoto, M. Sato, K. Takeya

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Abstract

Immune responses were examined after immunization with chicken erythrocytes (CRBC) in mice. Cytotoxicity of spleen cells was assessed by the release of 51Cr from labelled cells. (1) At early stages (day 4-7) after primary intraperitoneal immunization, direct cytotoxicity of spleen cells was raised efficiently in C57BL/6 and AKR mice, but not in C3H/He, SL and DDD mice. Delayed hypersensitivity and antibody production were raised to almost the same extent in all the strains at such periods. (2) Effector cells in direct cytotoxicity were θ-positive and IgG-positive, and glass-nonadherent and Nylon wool column-adherent. Effector cells in antibody-dependent cell-mediated cytotoxicity in the presence of antibody to CRBC were elmininated by treatment with anti-IgG serum but not by treatment with anti-θ serum. (3) Cytotoxicity and antibody production were raised efficiently after intraperitoneal or intravenous immunization, but not after footpad immunization. On the other hand, delayed hypersensitivity developed most efficiently after footpad immunization.

Original languageEnglish
Pages (from-to)895-905
Number of pages11
JournalImmunology
Volume33
Issue number6
Publication statusPublished - 1977

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Chickens
Immunization
Erythrocytes
T-Lymphocytes
Delayed Hypersensitivity
Antibody Formation
Spleen
Inbred AKR Mouse
Antibody-Dependent Cell Cytotoxicity
Dichlorodiphenyldichloroethane
Wool
Nylons
Serum
Inbred C57BL Mouse
Glass
Immunoglobulin G
Antibodies
Therapeutics

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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Direct cytotoxicity against chicken erythrocytes in mice. I. Fundamental nature of T cell-mediated cytotoxicity. / Kubo, Chiharu; Nomoto, K.; Sato, M.; Takeya, K.

In: Immunology, Vol. 33, No. 6, 1977, p. 895-905.

Research output: Contribution to journalArticle

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AB - Immune responses were examined after immunization with chicken erythrocytes (CRBC) in mice. Cytotoxicity of spleen cells was assessed by the release of 51Cr from labelled cells. (1) At early stages (day 4-7) after primary intraperitoneal immunization, direct cytotoxicity of spleen cells was raised efficiently in C57BL/6 and AKR mice, but not in C3H/He, SL and DDD mice. Delayed hypersensitivity and antibody production were raised to almost the same extent in all the strains at such periods. (2) Effector cells in direct cytotoxicity were θ-positive and IgG-positive, and glass-nonadherent and Nylon wool column-adherent. Effector cells in antibody-dependent cell-mediated cytotoxicity in the presence of antibody to CRBC were elmininated by treatment with anti-IgG serum but not by treatment with anti-θ serum. (3) Cytotoxicity and antibody production were raised efficiently after intraperitoneal or intravenous immunization, but not after footpad immunization. On the other hand, delayed hypersensitivity developed most efficiently after footpad immunization.

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