Abstract
Thermal lensing (TL) permits ultrasensitive measurements of optical absorption of analytes in very small volumes. Separation-detection conditions of non-labeled amino acids with micro-HPLC/UV absorption detector are optimized, and direct determination of non-labeled amino acids by micro-HPLC/UV-excitation TL detection in a gradient elution is successfully demonstrated. Non-labeled amol-level amino acids is detectable with the TL detection system, which has thousand times better sensitivity than a conventional UV detector.
| Original language | English |
|---|---|
| Article number | 012122 |
| Journal | Journal of Physics: Conference Series |
| Volume | 214 |
| DOIs | |
| Publication status | Published - 2010 |
All Science Journal Classification (ASJC) codes
- Physics and Astronomy(all)
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Katae, H., Hirashima, S., & Harata, A. (2010). Direct detection of gradient-eluted non-labeled amino acids using micro-HPLC with ultraviolet thermal lensing. Journal of Physics: Conference Series, 214, [012122]. https://doi.org/10.1088/1742-6596/214/1/012122