Direct detection of gradient-eluted non-labeled amino acids using micro-HPLC with ultraviolet thermal lensing

H. Katae, S. Hirashima, Akira Harata

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Thermal lensing (TL) permits ultrasensitive measurements of optical absorption of analytes in very small volumes. Separation-detection conditions of non-labeled amino acids with micro-HPLC/UV absorption detector are optimized, and direct determination of non-labeled amino acids by micro-HPLC/UV-excitation TL detection in a gradient elution is successfully demonstrated. Non-labeled amol-level amino acids is detectable with the TL detection system, which has thousand times better sensitivity than a conventional UV detector.

    Original languageEnglish
    Article number012122
    JournalJournal of Physics: Conference Series
    Volume214
    DOIs
    Publication statusPublished - Jan 1 2010

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    thermal lensing
    amino acids
    gradients
    elution
    detectors
    optical absorption
    sensitivity
    excitation

    All Science Journal Classification (ASJC) codes

    • Physics and Astronomy(all)

    Cite this

    Direct detection of gradient-eluted non-labeled amino acids using micro-HPLC with ultraviolet thermal lensing. / Katae, H.; Hirashima, S.; Harata, Akira.

    In: Journal of Physics: Conference Series, Vol. 214, 012122, 01.01.2010.

    Research output: Contribution to journalArticle

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