TY - JOUR
T1 - Direct detection of gradient-eluted non-labeled amino acids using micro-HPLC with ultraviolet thermal lensing
AU - Katae, H.
AU - Hirashima, S.
AU - Harata, A.
PY - 2010
Y1 - 2010
N2 - Thermal lensing (TL) permits ultrasensitive measurements of optical absorption of analytes in very small volumes. Separation-detection conditions of non-labeled amino acids with micro-HPLC/UV absorption detector are optimized, and direct determination of non-labeled amino acids by micro-HPLC/UV-excitation TL detection in a gradient elution is successfully demonstrated. Non-labeled amol-level amino acids is detectable with the TL detection system, which has thousand times better sensitivity than a conventional UV detector.
AB - Thermal lensing (TL) permits ultrasensitive measurements of optical absorption of analytes in very small volumes. Separation-detection conditions of non-labeled amino acids with micro-HPLC/UV absorption detector are optimized, and direct determination of non-labeled amino acids by micro-HPLC/UV-excitation TL detection in a gradient elution is successfully demonstrated. Non-labeled amol-level amino acids is detectable with the TL detection system, which has thousand times better sensitivity than a conventional UV detector.
UR - http://www.scopus.com/inward/record.url?scp=77950684098&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77950684098&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/214/1/012122
DO - 10.1088/1742-6596/214/1/012122
M3 - Article
AN - SCOPUS:77950684098
VL - 214
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
SN - 1742-6588
M1 - 012122
ER -