Direct excitation of inhibitory interneurons by extracellular ATP mediated by P2Y1 receptors in the hippocampal slice

Masahito Kawamura, Christian Gachet, Kazuhide Inoue, Fusao Kato

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Abstract

ATP is an important cell-to-cell signaling molecule mediating the interactions between astrocytes and neurons in the CNS. In the hippocampal slices, ATP suppresses excitatory transmission mostly through activation of adenosine A1 receptors, because the ectoenzyme activity for the extracellular breakdown of ATP to adenosine is high in slice preparations in contrast to culture environments. Because the hippocampus is also rich in the expression of P2 receptors activated specifically by ATP, we examined whether ATP modulates neuronal excitability in the acute slice preparations independently of adenosine receptors. Although ATP decreased the frequency of spontaneously occurring EPSCs in the CA3 pyramidal neurons through activation of adenosine A1 receptors, ATP concurrently increased the frequency of IPSCs in a manner dependent on action potential generation. This effect was mediated by P2Y1 receptors because (1) 2-methylthio-ATP (2meSATP) was the most potent agonist, (2) 2′-deoxy-N6-methyladenosine- 3′,5′-bisphosphate diammonium (MRS2179) abolished this effect, and (3) this increase in IPSC frequency was not observed in the transgenic mice lacking P2Y1 receptor proteins. Application of 2meSATP elicited MRS2179-sensitive time- and voltage-dependent inward currents in the interneurons, which depolarized the cell to firing threshold. Also, it increased [Ca2+]i in both astrocytes and interneurons, but, unlike the former effect, the latter was entirely dependent on Ca2+ entry. Thus, in hippocampal slices, in addition to activating A1 receptors of the excitatory terminals after being converted to adenosine, ATP activates P2Y1 receptors in the interneurons, which is linked to activation of unidentified excitatory conductance, through mechanisms distinct from those in the astrocytes.

Original languageEnglish
Pages (from-to)10835-10845
Number of pages11
JournalJournal of Neuroscience
Volume24
Issue number48
DOIs
Publication statusPublished - Dec 1 2004

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Purinergic P2Y1 Receptors
Interneurons
Adenosine Triphosphate
Astrocytes
Adenosine A1 Receptors
Adenosine
Purinergic P1 Receptors
Pyramidal Cells
Transgenic Mice
Action Potentials
Hippocampus
Neurons

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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Direct excitation of inhibitory interneurons by extracellular ATP mediated by P2Y1 receptors in the hippocampal slice. / Kawamura, Masahito; Gachet, Christian; Inoue, Kazuhide; Kato, Fusao.

In: Journal of Neuroscience, Vol. 24, No. 48, 01.12.2004, p. 10835-10845.

Research output: Contribution to journalArticle

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