Direct formation of giant unilamellar vesicles from microparticles of polyion complexes and investigation of their properties using a microfluidic chamber

Hidehiro Oana, Mutsuki Morinaga, Akihiro Kishimura, Kazunori Kataoka, Masao Washizu

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Although hollow microscopic capsules have a variety of potential biomedical applications, reports of organic-solvent-free methods for their preparation are rather limited. Herein, a novel approach is demonstrated for organic-solvent-free preparation of giant unilamellar vesicles utilizing the unique response of polyion complexes (PICs) to changes in additive salt concentration. A microfluidic device consisting of a main channel bearing side pockets that work as microscale reaction chambers is designed for facilitating the preparation process under an optical microscope. With this device, real-time observation of morphological transformation of individual PIC microparticles is carried out during rapid reduction of the additive salt concentration and direct formation of giant vesicles from PIC microparticles is shown. There is a quasilinear relationship between the surface areas of the formed vesicles and the volumes of the PIC microparticles, and the thickness of the vesicle membrane estimated by the relationship is indicative of the formation of a uniform unilamellar structure of the PIC membrane. Furthermore, detailed properties of the formed PIC vesicles with regard to salt response, loading of guest molecules, and permeability of the PIC membrane with/without modification of the PIC membrane by cross-linking are investigated using the microfluidic chamber. Thus, the usefulness of the microfluidic chamber for visualization and investigation of dynamic responses of microscale soft materials during changes in surrounding conditions is also demonstrated.

Original languageEnglish
Pages (from-to)5448-5458
Number of pages11
JournalSoft Matter
Volume9
Issue number22
DOIs
Publication statusPublished - Jun 14 2013

Fingerprint

Unilamellar Liposomes
microparticles
Microfluidics
chambers
membranes
Membranes
Salts
salts
microbalances
Organic solvents
preparation
Bearings (structural)
microfluidic devices
capsules
optical microscopes
dynamic response
Capsules
Dynamic response
hollow
permeability

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Condensed Matter Physics

Cite this

Direct formation of giant unilamellar vesicles from microparticles of polyion complexes and investigation of their properties using a microfluidic chamber. / Oana, Hidehiro; Morinaga, Mutsuki; Kishimura, Akihiro; Kataoka, Kazunori; Washizu, Masao.

In: Soft Matter, Vol. 9, No. 22, 14.06.2013, p. 5448-5458.

Research output: Contribution to journalArticle

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