Direct interaction of lignin and lignin peroxidase from phanerochaete chrysosporium

Toru Johjima, Noriyuki Itoh, Mari Kabuto, Fusayo Tokimura, Tamon Nakagawa, Hiroyuki Wariishi, Hiroo Tanaka

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Abstract

Binding properties of lignin peroxidase (LiP) from the basidiomycete Phanerochaete chrysosporium against a synthetic lignin (dehydrogenated polymerizate, DHP) were studied with a resonant mirror biosensor. Among several ligninolytic enzymes, only LiP specifically binds to DHP. Kinetic analysis revealed that the binding was reversible, and that the dissociation equilibrium constant was 330 μM. The LiP-DHP interaction was controlled by the ionization group with a pK(a) of 5.3, strongly suggesting that a specific amino acid residue plays a role in lignin binding. A one-electron transfer from DHP to oxidized intermediates LiP compounds I and II (LiPI and LiPII) was characterized by using a stopped-flow technique, showing that binding interactions of DHP with LiPI and LiPII led to saturation kinetics. The dissociation equilibrium constants for LiPI-DHP and LiPII-DHP interactions were calculated to be 350 and 250 μM, and the first-order rate constants for electron transfer from DHP to LiPI and to LiPII were calculated to be 46 and 16 s-1, respectively. These kinetic and spectral studies strongly suggest that LiP is capable of oxidizing lignin directly at the protein surface by a long-range electron transfer process. A close look at the crystal structure suggested that LiP possesses His-239 as a possible lignin-binding site on the surface, which is linked to Asp-238. This Asp residue is hydrogen-bonded to the proximal His-176. This HisAsp-proximal-His motif would be a possible electron transfer route to oxidize polymeric lignin.

Original languageEnglish
Pages (from-to)1989-1994
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number5
DOIs
Publication statusPublished - Mar 2 1999

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Phanerochaete
Lignin
Electrons
Basidiomycota
Biosensing Techniques
Viperidae
lignin peroxidase
Hydrogen
Membrane Proteins
Binding Sites
Amino Acids
Enzymes

All Science Journal Classification (ASJC) codes

  • General

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Direct interaction of lignin and lignin peroxidase from phanerochaete chrysosporium. / Johjima, Toru; Itoh, Noriyuki; Kabuto, Mari; Tokimura, Fusayo; Nakagawa, Tamon; Wariishi, Hiroyuki; Tanaka, Hiroo.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, No. 5, 02.03.1999, p. 1989-1994.

Research output: Contribution to journalArticle

Johjima, Toru ; Itoh, Noriyuki ; Kabuto, Mari ; Tokimura, Fusayo ; Nakagawa, Tamon ; Wariishi, Hiroyuki ; Tanaka, Hiroo. / Direct interaction of lignin and lignin peroxidase from phanerochaete chrysosporium. In: Proceedings of the National Academy of Sciences of the United States of America. 1999 ; Vol. 96, No. 5. pp. 1989-1994.
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