Direct light-up of cAMP derivatives in living cells by click reactions

Kenichiro Ito; Hongshan Liu; Makoto Komiyama; Tetsuya Hayashi; Yan Xu

doi:10.3390/molecules181012909

Direct light-up of cAMP derivatives in living cells by click reactions

Kenichiro Ito, Hongshan Liu, Makoto Komiyama, Tetsuya Hayashi, Yan Xu

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

8-Azidoadenosine 3′,5′-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target and difluorinated cyclooctyne (DIFO) reagent as a probe. The azide-alkyne cycloaddition reaction between 8-azido cAMP and DIFO induces fluorescence in 8-azido cAMP. The fluorescence emission serves as a way to probe 8-azido cAMP in cells.

Original language	English
Pages (from-to)	12909-12915
Number of pages	7
Journal	Molecules
Volume	18
Issue number	10
DOIs	https://doi.org/10.3390/molecules181012909
Publication status	Published - Oct 2013
Externally published	Yes

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry

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10.3390/molecules181012909

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title = "Direct light-up of cAMP derivatives in living cells by click reactions",

abstract = "8-Azidoadenosine 3′,5′-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target and difluorinated cyclooctyne (DIFO) reagent as a probe. The azide-alkyne cycloaddition reaction between 8-azido cAMP and DIFO induces fluorescence in 8-azido cAMP. The fluorescence emission serves as a way to probe 8-azido cAMP in cells.",

author = "Kenichiro Ito and Hongshan Liu and Makoto Komiyama and Tetsuya Hayashi and Yan Xu",

year = "2013",

month = oct,

doi = "10.3390/molecules181012909",

language = "English",

volume = "18",

pages = "12909--12915",

journal = "Molecules",

issn = "1420-3049",

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T1 - Direct light-up of cAMP derivatives in living cells by click reactions

AU - Ito, Kenichiro

AU - Liu, Hongshan

AU - Komiyama, Makoto

AU - Hayashi, Tetsuya

AU - Xu, Yan

PY - 2013/10

Y1 - 2013/10

N2 - 8-Azidoadenosine 3′,5′-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target and difluorinated cyclooctyne (DIFO) reagent as a probe. The azide-alkyne cycloaddition reaction between 8-azido cAMP and DIFO induces fluorescence in 8-azido cAMP. The fluorescence emission serves as a way to probe 8-azido cAMP in cells.

AB - 8-Azidoadenosine 3′,5′-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target and difluorinated cyclooctyne (DIFO) reagent as a probe. The azide-alkyne cycloaddition reaction between 8-azido cAMP and DIFO induces fluorescence in 8-azido cAMP. The fluorescence emission serves as a way to probe 8-azido cAMP in cells.

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U2 - 10.3390/molecules181012909

DO - 10.3390/molecules181012909

M3 - Article

C2 - 24141242

AN - SCOPUS:84886628626

SN - 1420-3049

VL - 18

SP - 12909

EP - 12915

JO - Molecules

JF - Molecules

IS - 10

ER -

Direct light-up of cAMP derivatives in living cells by click reactions

Abstract

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