Direct light-up of cAMP derivatives in living cells by click reactions

Kenichiro Ito, Hongshan Liu, Makoto Komiyama, Tetsuya Hayashi, Yan Xu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

8-Azidoadenosine 3′,5′-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target and difluorinated cyclooctyne (DIFO) reagent as a probe. The azide-alkyne cycloaddition reaction between 8-azido cAMP and DIFO induces fluorescence in 8-azido cAMP. The fluorescence emission serves as a way to probe 8-azido cAMP in cells.

Original languageEnglish
Pages (from-to)12909-12915
Number of pages7
JournalMolecules
Volume18
Issue number10
DOIs
Publication statusPublished - Oct 1 2013

Fingerprint

Fluorescence
Cells
Derivatives
Light
fluorescence
Alkynes
Azides
Cycloaddition
Cycloaddition Reaction
cycloaddition
alkynes
probes
reagents
Molecules
cells
molecules

All Science Journal Classification (ASJC) codes

  • Organic Chemistry

Cite this

Direct light-up of cAMP derivatives in living cells by click reactions. / Ito, Kenichiro; Liu, Hongshan; Komiyama, Makoto; Hayashi, Tetsuya; Xu, Yan.

In: Molecules, Vol. 18, No. 10, 01.10.2013, p. 12909-12915.

Research output: Contribution to journalArticle

Ito, Kenichiro ; Liu, Hongshan ; Komiyama, Makoto ; Hayashi, Tetsuya ; Xu, Yan. / Direct light-up of cAMP derivatives in living cells by click reactions. In: Molecules. 2013 ; Vol. 18, No. 10. pp. 12909-12915.
@article{b748ea4661c24f24815a7519ada2b73c,
title = "Direct light-up of cAMP derivatives in living cells by click reactions",
abstract = "8-Azidoadenosine 3′,5′-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target and difluorinated cyclooctyne (DIFO) reagent as a probe. The azide-alkyne cycloaddition reaction between 8-azido cAMP and DIFO induces fluorescence in 8-azido cAMP. The fluorescence emission serves as a way to probe 8-azido cAMP in cells.",
author = "Kenichiro Ito and Hongshan Liu and Makoto Komiyama and Tetsuya Hayashi and Yan Xu",
year = "2013",
month = "10",
day = "1",
doi = "10.3390/molecules181012909",
language = "English",
volume = "18",
pages = "12909--12915",
journal = "Molecules",
issn = "1420-3049",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "10",

}

TY - JOUR

T1 - Direct light-up of cAMP derivatives in living cells by click reactions

AU - Ito, Kenichiro

AU - Liu, Hongshan

AU - Komiyama, Makoto

AU - Hayashi, Tetsuya

AU - Xu, Yan

PY - 2013/10/1

Y1 - 2013/10/1

N2 - 8-Azidoadenosine 3′,5′-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target and difluorinated cyclooctyne (DIFO) reagent as a probe. The azide-alkyne cycloaddition reaction between 8-azido cAMP and DIFO induces fluorescence in 8-azido cAMP. The fluorescence emission serves as a way to probe 8-azido cAMP in cells.

AB - 8-Azidoadenosine 3′,5′-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target and difluorinated cyclooctyne (DIFO) reagent as a probe. The azide-alkyne cycloaddition reaction between 8-azido cAMP and DIFO induces fluorescence in 8-azido cAMP. The fluorescence emission serves as a way to probe 8-azido cAMP in cells.

UR - http://www.scopus.com/inward/record.url?scp=84886628626&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84886628626&partnerID=8YFLogxK

U2 - 10.3390/molecules181012909

DO - 10.3390/molecules181012909

M3 - Article

C2 - 24141242

AN - SCOPUS:84886628626

VL - 18

SP - 12909

EP - 12915

JO - Molecules

JF - Molecules

SN - 1420-3049

IS - 10

ER -