Direct Monitoring of γ-Glutamyl Transpeptidase Activity In Vivo Using a Hyperpolarized13C-Labeled Molecular Probe

Tatsuya Nishihara, Hikari A.I. Yoshihara, Hiroshi Nonaka, Yoichi Takakusagi, Fuminori Hyodo, Kazuhiro Ichikawa, Emine Can, Jessica A.M. Bastiaansen, Yuhei Takado, Arnaud Comment, Shinsuke Sando

    Research output: Contribution to journalArticlepeer-review

    21 Citations (Scopus)

    Abstract

    The γ-glutamyl transpeptidase (GGT) enzyme plays a central role in glutathione homeostasis. Direct detection of GGT activity could provide critical information for the diagnosis of several pathologies. We propose a new molecular probe, γ-Glu-[1-13C]Gly, for monitoring GGT activity in vivo by hyperpolarized (HP)13C magnetic resonance (MR). The properties of γ-Glu-[1-13C]Gly are suitable for in vivo HP13C metabolic analysis since the chemical shift between γ-Glu-[1-13C]Gly and its metabolic product, [1-13C]Gly, is large (4.3 ppm) and the T1of both compounds is relatively long (30 s and 45 s, respectively, in H2O at 9.4 T). We also demonstrate that γ-Glu-[1-13C]Gly is highly sensitive to in vivo modulation of GGT activity induced by the inhibitor acivicin.

    Original languageEnglish
    Pages (from-to)10626-10629
    Number of pages4
    JournalAngewandte Chemie - International Edition
    Volume55
    Issue number36
    DOIs
    Publication statusPublished - Aug 26 2016

    All Science Journal Classification (ASJC) codes

    • Catalysis
    • Chemistry(all)

    Fingerprint

    Dive into the research topics of 'Direct Monitoring of γ-Glutamyl Transpeptidase Activity In Vivo Using a Hyperpolarized<sup>13</sup>C-Labeled Molecular Probe'. Together they form a unique fingerprint.

    Cite this