Direct Monitoring of γ-Glutamyl Transpeptidase Activity In Vivo Using a Hyperpolarized13C-Labeled Molecular Probe

Tatsuya Nishihara, Hikari A.I. Yoshihara, Hiroshi Nonaka, Yoichi Takakusagi, Fuminori Hyodo, Kazuhiro Ichikawa, Emine Can, Jessica A.M. Bastiaansen, Yuhei Takado, Arnaud Comment, Shinsuke Sando

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16 Citations (Scopus)

Abstract

The γ-glutamyl transpeptidase (GGT) enzyme plays a central role in glutathione homeostasis. Direct detection of GGT activity could provide critical information for the diagnosis of several pathologies. We propose a new molecular probe, γ-Glu-[1-13C]Gly, for monitoring GGT activity in vivo by hyperpolarized (HP)13C magnetic resonance (MR). The properties of γ-Glu-[1-13C]Gly are suitable for in vivo HP13C metabolic analysis since the chemical shift between γ-Glu-[1-13C]Gly and its metabolic product, [1-13C]Gly, is large (4.3 ppm) and the T1of both compounds is relatively long (30 s and 45 s, respectively, in H2O at 9.4 T). We also demonstrate that γ-Glu-[1-13C]Gly is highly sensitive to in vivo modulation of GGT activity induced by the inhibitor acivicin.

Original languageEnglish
Pages (from-to)10626-10629
Number of pages4
JournalAngewandte Chemie - International Edition
Volume55
Issue number36
DOIs
Publication statusPublished - Jan 1 2016

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)

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    Nishihara, T., Yoshihara, H. A. I., Nonaka, H., Takakusagi, Y., Hyodo, F., Ichikawa, K., Can, E., Bastiaansen, J. A. M., Takado, Y., Comment, A., & Sando, S. (2016). Direct Monitoring of γ-Glutamyl Transpeptidase Activity In Vivo Using a Hyperpolarized13C-Labeled Molecular Probe. Angewandte Chemie - International Edition, 55(36), 10626-10629. https://doi.org/10.1002/anie.201603731