Direct monitoring of EcoRII restriction enzyme reactions on a 27 MHz quartz-crystal microbalance.

Shuntaro Takahashi, Hisao Matsuno, Hiroyuki Furusawa, Yoshio Okahata

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

EcoRII is a type IIe restriction endonuclease characterized by a highly cooperative reaction mechanism that depends on simultaneous binding of the dimeric enzyme molecule to two copies of its recognition site. Gaining insights about reaction property of EcoRII binding to its recognition site and effect of divalent metal ion on DNA cleavage process, a DNA-immobilized quartz crystal microbalance (QCM), which enables real-time monitoring both the binding of enzyme and the cleavage reaction on DNA strands as mass changes, was utilized.

Original languageEnglish
Pages (from-to)71-72
Number of pages2
JournalNucleic acids research. Supplement (2001)
Issue number2
DOIs
Publication statusPublished - 2002
Externally publishedYes

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