Direct observation of ATP-induced conformational changes in single P2X 4 receptors

Youichi Shinozaki, Koji Sumitomo, Makoto Tsuda, Schuichi Koizumi, Kazuhide Inoue, Keiichi Torimitsu

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

The ATP-gated P2X4 receptor is a cation channel, which is important in various pathophysiological events. The architecture of the P2X 4 receptor in the activated state and how to change its structure in response to ATP binding are not fully understood. Here, we analyze the architecture and ATP-induced structural changes in P2X4 receptors using fast-scanning atomic force microscopy (AFM). AFM images of the membrane-dissociated and membrane-inserted forms of P2X4 receptors and a functional analysis revealed that P2X4 receptors have an upward orientation on mica but lean to one side. Time-lapse imaging of the ATP-induced structural changes in P2X4 receptors revealed two different forms of activated structures under 0 Ca2+ conditions, namely a trimer structure and a pore dilation-like tripartite structure. A dye uptake measurement demonstrated that ATP-activated P2X4 receptors display pore dilation in the absence of Ca2+. With Ca2+, the P2X4 receptors exhibited only a disengaged trimer and no dye uptake was observed. Thus our data provide a new insight into ATP-induced structural changes in P2X4 receptors that correlate with pore dynamics.

Original languageEnglish
Article numbere1000103
JournalPLoS Biology
Volume7
Issue number5
DOIs
Publication statusPublished - May 1 2009

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Purinergic P2X4 Receptors
Adenosine Triphosphate
Observation
receptors
Atomic Force Microscopy
atomic force microscopy
calcium
Dilatation
Atomic force microscopy
Coloring Agents
dyes
Time-Lapse Imaging
Membranes
Functional analysis
mica
Cations
cations
Scanning
Imaging techniques

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Direct observation of ATP-induced conformational changes in single P2X 4 receptors. / Shinozaki, Youichi; Sumitomo, Koji; Tsuda, Makoto; Koizumi, Schuichi; Inoue, Kazuhide; Torimitsu, Keiichi.

In: PLoS Biology, Vol. 7, No. 5, e1000103, 01.05.2009.

Research output: Contribution to journalArticle

Shinozaki, Youichi ; Sumitomo, Koji ; Tsuda, Makoto ; Koizumi, Schuichi ; Inoue, Kazuhide ; Torimitsu, Keiichi. / Direct observation of ATP-induced conformational changes in single P2X 4 receptors. In: PLoS Biology. 2009 ; Vol. 7, No. 5.
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