Direct stimulation of cellular immune response via TLR2 signaling triggered by contact with hybrid glyco-biointerfaces composed of chitohexaose and cellohexaose

Mayumi Hatakeyama, Fumi Nakada, Hirofumi Ichinose, Takuya Kitaoka

Research output: Contribution to journalArticle

Abstract

Recognition of carbohydrates by cell receptors activates various cell signaling processes. In this report, hybrid self-assembled monolayers (SAMs) of chitohexaose (βGlcNAc6) and cellohexaose (βGlc6) are prepared to induce a highly sensitive, glyco-density-dependent inflammatory response by HEK293 cells expressing toll-like receptor 2 (TLR2), which recognizes oligo-βGlcNAc moieties. Thiosemicarbazide-labeled βGlcNAc6 and βGlc6, as a bio-signal inducer and a spacer molecule, respectively, were immobilized on a gold substrate via spontaneous chemisorption to control the βGlcNAc6 density on the SAM surface. The βGlcNAc6 density ranged from 0 to 0.65 chains nm –2 . The inflammatory response of HEK293 cells varied as a function of the surface glyco-density, and the hybrid SAM with 0.146 chains nm –2 of βGlcNAc6 induced a stronger response than pure βGlcNAc6-SAM with 0.654 chains nm –2 . Thus, direct activation of TLR2-mediated cellular stimulation triggered by as-designed glyco-biointerfaces was possible through a mutual interaction between the fittingly βGlcNAc6 moieties assembled on hybrid glyco-SAMs and TLR2 on the surface of HEK293 cells.

Original languageEnglish
Pages (from-to)517-522
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Volume175
DOIs
Publication statusPublished - Mar 1 2019

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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