Direct vasodilative effect of FK506 on porcine mesenteric artery in small bowel transplantation

Junko Akiyoshi, Satoshi Ieiri, Takanori Nakatsuji, Tomoaki Taguchi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Tacrolimus (FK506) is widely used as an immunosuppressive drug in small bowel transplantation. However, its precise effects on the vascular tone of the transplanted organ have not been studied. This study aimed to clarify the effects of FK506 on the porcine mesenteric artery. Methods: The effects of FK506 on the changes in cytosolic Ca2+ concentration ([Ca2+]i) and force using fura-2 fluorometry were investigated in mesenteric arterial strips of the porcine small intestine. The effects of FK506 on the activity of voltage-dependent Ca2+ channels and receptor-operated Ca2+ channels using high K+ (118 mmol/L K+) depolarization and thromboxane A2 analog (U46619) stimulation were also examined. Results: FK506 inhibited the force development induced by 118 mmol/L K+ depolarization and 1 μmol/L U46619 stimulation in a concentration-dependent manner. The extent of inhibition of this contraction was greater than that of the K+-induced contraction, and its inhibitory potency was about 10-fold. FK506 (10 μmol/L) inhibited the increases in [Ca2+]i (24.9% ± 7.4%) and the force development (52.0% ± 5.6%) induced by 1 μmol/L U46619, respectively. Conclusions: FK506 induces arterial relaxation by decreasing [Ca2+]i. Pretreatment of a graft with FK506 may reduce the risk of vasospasm, ischemia-reperfusion injury, and thrombosis in small bowel transplantation.

Original languageEnglish
Pages (from-to)2322-2326
Number of pages5
JournalJournal of Pediatric Surgery
Volume44
Issue number12
DOIs
Publication statusPublished - Dec 1 2009

Fingerprint

Mesenteric Arteries
Tacrolimus
Swine
Transplantation
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
Fluorometry
Thromboxane A2
Immunosuppressive Agents
Reperfusion Injury
Small Intestine
Blood Vessels
Thrombosis
Transplants

All Science Journal Classification (ASJC) codes

  • Surgery
  • Pediatrics, Perinatology, and Child Health

Cite this

Direct vasodilative effect of FK506 on porcine mesenteric artery in small bowel transplantation. / Akiyoshi, Junko; Ieiri, Satoshi; Nakatsuji, Takanori; Taguchi, Tomoaki.

In: Journal of Pediatric Surgery, Vol. 44, No. 12, 01.12.2009, p. 2322-2326.

Research output: Contribution to journalArticle

Akiyoshi, Junko ; Ieiri, Satoshi ; Nakatsuji, Takanori ; Taguchi, Tomoaki. / Direct vasodilative effect of FK506 on porcine mesenteric artery in small bowel transplantation. In: Journal of Pediatric Surgery. 2009 ; Vol. 44, No. 12. pp. 2322-2326.
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