Directed aggregation and fusion of lipid vesicles induced by DNA-surfactants

Tatsuo Maruyama, Hiroshi Yamamura, Mai Hiraki, Yoshinori Kemori, Harumi Takata, Masahiro Goto

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We investigated DNA-directed aggregation of vesicles using DNA-surfactants. Following tethering of single-stranded DNA oligonucleotides to vesicles using DNA-surfactant, the tethered vesicles were assembled with other vesicles bearing complementary strands. The vesicle aggregation was strongly affected by the salt concentration and by temperature according to the characteristics of DNA hybridization. Restriction enzyme, which can hydrolyze the double-stranded DNA used in the present study, dissociated the vesicle aggregates. Exploration using fluorescently labeled vesicles suggested that the DNA-directed vesicle aggregation took place in a sequence-specific manner through DNA-duplex formation. Interestingly, the DNA-directed aggregation using short DNA-surfactant induced the fusion of vesicles to produce giant vesicles, resulting in an enzymatic reaction in the giant vesicle.

Original languageEnglish
Pages (from-to)119-124
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Volume66
Issue number1
DOIs
Publication statusPublished - Oct 1 2008

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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