TY - JOUR
T1 - Directed aggregation and fusion of lipid vesicles induced by DNA-surfactants
AU - Maruyama, Tatsuo
AU - Yamamura, Hiroshi
AU - Hiraki, Mai
AU - Kemori, Yoshinori
AU - Takata, Harumi
AU - Goto, Masahiro
N1 - Funding Information:
This study was financially supported by a Grant-in-Aid for Scientific Research (No. 17656271) from the Ministry of Education, Science Sports and Culture of Japan. We thank Nippon Fine Chemical Co. for kindly providing POPC.
PY - 2008/10/1
Y1 - 2008/10/1
N2 - We investigated DNA-directed aggregation of vesicles using DNA-surfactants. Following tethering of single-stranded DNA oligonucleotides to vesicles using DNA-surfactant, the tethered vesicles were assembled with other vesicles bearing complementary strands. The vesicle aggregation was strongly affected by the salt concentration and by temperature according to the characteristics of DNA hybridization. Restriction enzyme, which can hydrolyze the double-stranded DNA used in the present study, dissociated the vesicle aggregates. Exploration using fluorescently labeled vesicles suggested that the DNA-directed vesicle aggregation took place in a sequence-specific manner through DNA-duplex formation. Interestingly, the DNA-directed aggregation using short DNA-surfactant induced the fusion of vesicles to produce giant vesicles, resulting in an enzymatic reaction in the giant vesicle.
AB - We investigated DNA-directed aggregation of vesicles using DNA-surfactants. Following tethering of single-stranded DNA oligonucleotides to vesicles using DNA-surfactant, the tethered vesicles were assembled with other vesicles bearing complementary strands. The vesicle aggregation was strongly affected by the salt concentration and by temperature according to the characteristics of DNA hybridization. Restriction enzyme, which can hydrolyze the double-stranded DNA used in the present study, dissociated the vesicle aggregates. Exploration using fluorescently labeled vesicles suggested that the DNA-directed vesicle aggregation took place in a sequence-specific manner through DNA-duplex formation. Interestingly, the DNA-directed aggregation using short DNA-surfactant induced the fusion of vesicles to produce giant vesicles, resulting in an enzymatic reaction in the giant vesicle.
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U2 - 10.1016/j.colsurfb.2008.05.017
DO - 10.1016/j.colsurfb.2008.05.017
M3 - Article
C2 - 18617375
AN - SCOPUS:47249089013
SN - 0927-7765
VL - 66
SP - 119
EP - 124
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
IS - 1
ER -