Directly linked soluble IL-6 receptor-IL-6 fusion protein induces astrocyte differentiation from neuroepithelial cells via activation of STAT3

Takumi Takizawa, Makoto Yanagisawa, Wataru Ochiai, Kiyoshi Yasukawa, Takahiko Ishiguro, Kinichi Nakashima, Tetsuya Taga

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Signals of interleukin 6 (IL-6) are transduced by binding of IL-6 to its cell surface receptor (IL-6R) and subsequent association of the resultant IL-6/IL-6R complex with gp130, the signal transducing receptor component utilized in common by all the IL-6 family of cytokines. A soluble form of IL-6R (sIL-6R), which lacks transmembrane and cytoplasmic regions, retains the ability to bind IL-6 and signal through gp130. We show here that a fusion protein of sIL-6R and IL-6 without a polypeptide linker, termed FP6, induces differentiation of astrocytes from fetal mouse neuroepithelial cells as potently as a representative IL-6 family cytokine, leukaemia inhibitory factor (LIF). FP6 has a potential to activate a transcription factor, signal transducer and activator of transcription 3 (STAT3), and mitogen-activated protein kinases, ERK1 and ERK2, in these cells as does LIF. FP6 activates a promoter of the gene for an astrocytic marker, glial fibrillary acidic protein (GFAP), in neuroepithelial cells. This activation is virtually abolished by ectopic expression of a dominant-negative form of STAT3, or by introducing a point mutation into the STAT3 response element located in the GFAP promoter. These results suggest that FP6 induces astrocyte differentiation from neuroepithelial cells through STAT3 activation and that FP6 could be of use as a substitute for natural IL-6 family cytokines.

Original languageEnglish
Pages (from-to)272-279
Number of pages8
JournalCytokine
Volume13
Issue number5
DOIs
Publication statusPublished - Mar 7 2001

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Neuroepithelial Cells
STAT3 Transcription Factor
Astrocytes
Interleukin-6
Chemical activation
Leukemia Inhibitory Factor
Glial Fibrillary Acidic Protein
Cytokines
Cytokine Receptor gp130
interleukin 6-interleukin 6 receptor fusion protein, recombinant
Cell Surface Receptors
Response Elements
Mitogen-Activated Protein Kinases
Point Mutation
Transcriptional Activation
Transcription Factors
Fusion reactions
Genes
Association reactions
Peptides

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

Cite this

Directly linked soluble IL-6 receptor-IL-6 fusion protein induces astrocyte differentiation from neuroepithelial cells via activation of STAT3. / Takizawa, Takumi; Yanagisawa, Makoto; Ochiai, Wataru; Yasukawa, Kiyoshi; Ishiguro, Takahiko; Nakashima, Kinichi; Taga, Tetsuya.

In: Cytokine, Vol. 13, No. 5, 07.03.2001, p. 272-279.

Research output: Contribution to journalArticle

Takizawa, Takumi ; Yanagisawa, Makoto ; Ochiai, Wataru ; Yasukawa, Kiyoshi ; Ishiguro, Takahiko ; Nakashima, Kinichi ; Taga, Tetsuya. / Directly linked soluble IL-6 receptor-IL-6 fusion protein induces astrocyte differentiation from neuroepithelial cells via activation of STAT3. In: Cytokine. 2001 ; Vol. 13, No. 5. pp. 272-279.
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