Dis3, implicated in mitotic control, binds directly to Ran and enhances the GEF activity of RCC1

E. Noguchi, N. Hayashi, Y. Azuma, T. Seki, Masafumi Nakamura, N. Nakashima, M. Yanagida, X. He, U. Mueller, S. Sazer, T. Nishimoto

Research output: Contribution to journalArticle

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Abstract

Using the two-hybrid method, we isolated a Saccharomyces cerevisiae cDNA encoding a protein homologous to Schizosaccharomyces pombe protein Dis3sp, using as bait, human GTPase Ran. The DIS3 gene is essential for viability and complements S.pombe mutant dis3-54 which is defective in mitosis. Although Dis3sc has no homology to RanBP1, it bound directly to Ran and the S.cerevisiae Ran homologue Cnr1, but not to the S.cerevisiae RCC1 homologue Srm1. Upon binding to Ran with a 1:1 molar ratio, Dis3sc enhanced a nucleotide-releasing activity of RCC1 on Ran. In the presence of Dis3sc, the K(m) of RCC1 on Ran decreased by half, while the k(cat) as unchanged. In vivo, Dis3sp was present as oligomers of M(r) 670-200 kDa as previously reported, and the 200 kDa oligomer of Dis3sp was found to include Spit and Pim1, the S. pombe homologues of Ran and RCC1, respectively. Although the biological function of the heterotrimeric oligomer consisting of Dis3, Spi1 and Pim1 is unknown, our results indicate that Dis3 is a component of the RCC1-Ran pathway.

Original languageEnglish
Pages (from-to)5595-5605
Number of pages11
JournalEMBO Journal
Volume15
Issue number20
Publication statusPublished - Oct 15 1996

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Oligomers
Saccharomyces cerevisiae
Schizosaccharomyces
Schizosaccharomyces pombe Proteins
Two-Hybrid System Techniques
GTP Phosphohydrolases
Essential Genes
Mitosis
Yeast
Nucleotides
Complementary DNA
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Noguchi, E., Hayashi, N., Azuma, Y., Seki, T., Nakamura, M., Nakashima, N., ... Nishimoto, T. (1996). Dis3, implicated in mitotic control, binds directly to Ran and enhances the GEF activity of RCC1. EMBO Journal, 15(20), 5595-5605.

Dis3, implicated in mitotic control, binds directly to Ran and enhances the GEF activity of RCC1. / Noguchi, E.; Hayashi, N.; Azuma, Y.; Seki, T.; Nakamura, Masafumi; Nakashima, N.; Yanagida, M.; He, X.; Mueller, U.; Sazer, S.; Nishimoto, T.

In: EMBO Journal, Vol. 15, No. 20, 15.10.1996, p. 5595-5605.

Research output: Contribution to journalArticle

Noguchi, E, Hayashi, N, Azuma, Y, Seki, T, Nakamura, M, Nakashima, N, Yanagida, M, He, X, Mueller, U, Sazer, S & Nishimoto, T 1996, 'Dis3, implicated in mitotic control, binds directly to Ran and enhances the GEF activity of RCC1', EMBO Journal, vol. 15, no. 20, pp. 5595-5605.
Noguchi E, Hayashi N, Azuma Y, Seki T, Nakamura M, Nakashima N et al. Dis3, implicated in mitotic control, binds directly to Ran and enhances the GEF activity of RCC1. EMBO Journal. 1996 Oct 15;15(20):5595-5605.
Noguchi, E. ; Hayashi, N. ; Azuma, Y. ; Seki, T. ; Nakamura, Masafumi ; Nakashima, N. ; Yanagida, M. ; He, X. ; Mueller, U. ; Sazer, S. ; Nishimoto, T. / Dis3, implicated in mitotic control, binds directly to Ran and enhances the GEF activity of RCC1. In: EMBO Journal. 1996 ; Vol. 15, No. 20. pp. 5595-5605.
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AU - Nakashima, N.

AU - Yanagida, M.

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