Disappearance of the lymphoid system in Bcl-2 homozygous mutant chimeric mice

Kei Ichi Nakayama, Keiko Nakayama, Izumi Negishi, Keisuke Kuida, Yoichi Shinkai, Marjorie C. Louie, Larry E. Fields, Phillip J. Lucas, Valerie Stewart, Frederick W. Alt, Dennis Y. Loh

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Abstract

The bcl-2 proto-oncogene can prevent the death of many cell types. Mice were generated that were chimeric for the homozygous inactivation of bcl-2. Lymphocytes without Bcl-2 differentiated into phenotypically mature cells. However, in vitro, the mature T cells that lacked Bcl-2 had shorter life-spans and increased sensitivity to glucocorticoids and γ-irradiation. In contrast, stimulation of CD3 inhibited the death of these cells. T and B cells with no Bcl-2 disappeared from the bone marrow, thymus, and periphery by 4 weeks of age. Thus, Bcl-2 was dispensable for lymphocyte maturation, but was required for a stable immune system after birth.

Original languageEnglish
Pages (from-to)1584-1588
Number of pages5
JournalScience
Volume261
Issue number5128
DOIs
Publication statusPublished - Jan 1 1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

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    Nakayama, K. I., Nakayama, K., Negishi, I., Kuida, K., Shinkai, Y., Louie, M. C., Fields, L. E., Lucas, P. J., Stewart, V., Alt, F. W., & Loh, D. Y. (1993). Disappearance of the lymphoid system in Bcl-2 homozygous mutant chimeric mice. Science, 261(5128), 1584-1588. https://doi.org/10.1126/science.8372353