Abstract
The exposure of humans and experimental animals to certain industrial toxins such as acrylamide is known to cause nerve damage classified as axonopathy, but the mechanisms involved are poorly understood. Here we show that acrylamide induces morphological changes and tyrosine phosphorylation of focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (Pyk2), a member of the FAK subfamily, in human differentiating neuroblastoma SH-SY5Y cells. Furthermore, we identified a novel molecule designated 'compound-1' that inhibits the morphological and biochemical events. Daily oral administrations of the compound also effectively alleviated behavioral deficits in animals elicited by acrylamide in inclined plane testing, landing foot spread testing and rota-rod performance testing. The compound also effectively inhibited the biological and biochemical responses caused by another axonopathy inducer, colchicine, including tyrosine phosphorylation of Pyk2, formation of an 85-kDa poly(ADP-ribose)polymerase (PARP) fragment and apoptosis-associated induction of the NAPOR gene as well as neuronal cell death. Our findings not only provide insight into FAK and Pyk2 functions in neuronal cells, but may also be important in the development of therapeutic agents for peripheral neuropathy and neurodegeneration
| Original language | English |
|---|---|
| Pages (from-to) | 8-19 |
| Number of pages | 12 |
| Journal | Brain Research |
| Volume | 909 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Aug 3 2001 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
- Molecular Biology
- Clinical Neurology
- Developmental Biology
Cite this
Discovery of a novel compound : Insight into mechanisms for acrylamide-induced axonopathy and colchicine-induced apoptotic neuronal cell death. / Nakagawa-Yagi, Yuzo; Choi, Dong Kug; Ogane, Nobuo; Shimada, Shin Ichi; Seya, Motohide; Momoi, Takashi; Ito, Takashi; Sakaki, Yoshiyuki.
In: Brain Research, Vol. 909, No. 1-2, 03.08.2001, p. 8-19.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Discovery of a novel compound
T2 - Insight into mechanisms for acrylamide-induced axonopathy and colchicine-induced apoptotic neuronal cell death
AU - Nakagawa-Yagi, Yuzo
AU - Choi, Dong Kug
AU - Ogane, Nobuo
AU - Shimada, Shin Ichi
AU - Seya, Motohide
AU - Momoi, Takashi
AU - Ito, Takashi
AU - Sakaki, Yoshiyuki
PY - 2001/8/3
Y1 - 2001/8/3
N2 - The exposure of humans and experimental animals to certain industrial toxins such as acrylamide is known to cause nerve damage classified as axonopathy, but the mechanisms involved are poorly understood. Here we show that acrylamide induces morphological changes and tyrosine phosphorylation of focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (Pyk2), a member of the FAK subfamily, in human differentiating neuroblastoma SH-SY5Y cells. Furthermore, we identified a novel molecule designated 'compound-1' that inhibits the morphological and biochemical events. Daily oral administrations of the compound also effectively alleviated behavioral deficits in animals elicited by acrylamide in inclined plane testing, landing foot spread testing and rota-rod performance testing. The compound also effectively inhibited the biological and biochemical responses caused by another axonopathy inducer, colchicine, including tyrosine phosphorylation of Pyk2, formation of an 85-kDa poly(ADP-ribose)polymerase (PARP) fragment and apoptosis-associated induction of the NAPOR gene as well as neuronal cell death. Our findings not only provide insight into FAK and Pyk2 functions in neuronal cells, but may also be important in the development of therapeutic agents for peripheral neuropathy and neurodegeneration
AB - The exposure of humans and experimental animals to certain industrial toxins such as acrylamide is known to cause nerve damage classified as axonopathy, but the mechanisms involved are poorly understood. Here we show that acrylamide induces morphological changes and tyrosine phosphorylation of focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (Pyk2), a member of the FAK subfamily, in human differentiating neuroblastoma SH-SY5Y cells. Furthermore, we identified a novel molecule designated 'compound-1' that inhibits the morphological and biochemical events. Daily oral administrations of the compound also effectively alleviated behavioral deficits in animals elicited by acrylamide in inclined plane testing, landing foot spread testing and rota-rod performance testing. The compound also effectively inhibited the biological and biochemical responses caused by another axonopathy inducer, colchicine, including tyrosine phosphorylation of Pyk2, formation of an 85-kDa poly(ADP-ribose)polymerase (PARP) fragment and apoptosis-associated induction of the NAPOR gene as well as neuronal cell death. Our findings not only provide insight into FAK and Pyk2 functions in neuronal cells, but may also be important in the development of therapeutic agents for peripheral neuropathy and neurodegeneration
UR - http://www.scopus.com/inward/record.url?scp=0035800625&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035800625&partnerID=8YFLogxK
U2 - 10.1016/S0006-8993(01)02608-7
DO - 10.1016/S0006-8993(01)02608-7
M3 - Article
C2 - 11478917
AN - SCOPUS:0035800625
VL - 909
SP - 8
EP - 19
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1-2
ER -