Discovery of a novel flavivirus (Flaviviridae) from the horse fly, tabanus rufidens (Diptera: Tabanidae): The possible coevolutionary relationships between the classical insect-specific flaviviruses and host dipteran insects

Daisuke Kobayashi, Mamoru Watanabe, Astri Nur Faizah, Michael Amoa-Bosompem, Yukiko Higa, Yoshio Tsuda, Kyoko Sawabe, Haruhiko Isawa

Research output: Contribution to journalArticlepeer-review

Abstract

Tabanid flies (Tabanidae: Diptera) are common hematophagous insects known to transmit some pathogens mechanically or biologically to animals; they are widely distributed throughout the world. However, no tabanid-borne viruses, except mechanically transmitted viruses, have been reported to date. In this study, we conducted RNA virome analysis of several human-biting tabanid species in Japan, to discover and characterize viruses associated with tabanids. A novel flavivirus was encountered during the study in the Japanese horse fly, Tabanus rufidens (Bigot, 1887). The virus was detected only in T. rufidens, but not in other tabanid species, and as such was designated Tabanus rufidens flavivirus (TrFV). TrFV could not be isolated using a mammalian cell line and showed a closer phylogenetic relationship to the classical insect-specific flaviviruses (cISFs) rather than the vertebrate-infecting flaviviruses (VIFs), suggesting that it is a novel member of the cISFs. The first discovery of a cISF from Brachycera provides new insight into the evolutionary history and dynamics of flaviviruses.

Original languageEnglish
Pages (from-to)880-890
Number of pages11
JournalJournal of Medical Entomology
Volume58
Issue number2
DOIs
Publication statusPublished - Mar 1 2021
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

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