Discovery of a novel immunoglobulin heavy chain gene chimera from common carp (Cyprinus carpio L.)

Ram Savan, Azumi Aman, Miki Nakao, Hironobu Watanuki, Masahiro Sakai

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

In fish, two types of immunoglobulin heavy chain (IGH) genes, namely, IgM and IgD, have been cloned and characterized. Recently, a new IGH isotype specific to teleosts had been identified from zebra fish, rainbow trout, and fugu. In zebra fish, the domains of this new gene are present upstream of the μ region along the IGH locus. During this study, a novel IGH chimera (IgM-IgZ) has been discovered from common carp. The cloned cDNA encodes a typical leader peptide, a variable region, two constant regions, and a secretory tail. The first constant region is made up of the CH1 domain of carp IgM, while the second constant region shares a high similarity to the C H4 domain of the IgZ from zebrafish. Southern hybridization studies of the μ and ζ domains, conducted separately, revealed the presence of at least three copies of the respective genes, and μ and ζ domains might be present on the same loci, although far apart. Expression studies of the IGH genes suggest that there is an increase in chimeric immunoglobulin gene transcription when stimulated with lipopolysaccharide.

Original languageEnglish
Pages (from-to)458-463
Number of pages6
JournalImmunogenetics
Volume57
Issue number6
DOIs
Publication statusPublished - Jul 1 2005

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Immunoglobulin Heavy Chain Genes
Immunoglobulin Heavy Chains
Carps
Zebrafish
Immunoglobulin M
Takifugu
Immunoglobulin D
Immunoglobulin Genes
Oncorhynchus mykiss
Protein Sorting Signals
Genes
Lipopolysaccharides
Tail
Fishes
Complementary DNA

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

Discovery of a novel immunoglobulin heavy chain gene chimera from common carp (Cyprinus carpio L.). / Savan, Ram; Aman, Azumi; Nakao, Miki; Watanuki, Hironobu; Sakai, Masahiro.

In: Immunogenetics, Vol. 57, No. 6, 01.07.2005, p. 458-463.

Research output: Contribution to journalArticle

Savan, Ram ; Aman, Azumi ; Nakao, Miki ; Watanuki, Hironobu ; Sakai, Masahiro. / Discovery of a novel immunoglobulin heavy chain gene chimera from common carp (Cyprinus carpio L.). In: Immunogenetics. 2005 ; Vol. 57, No. 6. pp. 458-463.
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