Abstract
In fish, two types of immunoglobulin heavy chain (IGH) genes, namely, IgM and IgD, have been cloned and characterized. Recently, a new IGH isotype specific to teleosts had been identified from zebra fish, rainbow trout, and fugu. In zebra fish, the domains of this new gene are present upstream of the μ region along the IGH locus. During this study, a novel IGH chimera (IgM-IgZ) has been discovered from common carp. The cloned cDNA encodes a typical leader peptide, a variable region, two constant regions, and a secretory tail. The first constant region is made up of the CH1 domain of carp IgM, while the second constant region shares a high similarity to the C H4 domain of the IgZ from zebrafish. Southern hybridization studies of the μ and ζ domains, conducted separately, revealed the presence of at least three copies of the respective genes, and μ and ζ domains might be present on the same loci, although far apart. Expression studies of the IGH genes suggest that there is an increase in chimeric immunoglobulin gene transcription when stimulated with lipopolysaccharide.
| Original language | English |
|---|---|
| Pages (from-to) | 458-463 |
| Number of pages | 6 |
| Journal | Immunogenetics |
| Volume | 57 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jul 1 2005 |
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All Science Journal Classification (ASJC) codes
- Immunology
- Genetics
Cite this
Discovery of a novel immunoglobulin heavy chain gene chimera from common carp (Cyprinus carpio L.). / Savan, Ram; Aman, Azumi; Nakao, Miki; Watanuki, Hironobu; Sakai, Masahiro.
In: Immunogenetics, Vol. 57, No. 6, 01.07.2005, p. 458-463.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Discovery of a novel immunoglobulin heavy chain gene chimera from common carp (Cyprinus carpio L.)
AU - Savan, Ram
AU - Aman, Azumi
AU - Nakao, Miki
AU - Watanuki, Hironobu
AU - Sakai, Masahiro
PY - 2005/7/1
Y1 - 2005/7/1
N2 - In fish, two types of immunoglobulin heavy chain (IGH) genes, namely, IgM and IgD, have been cloned and characterized. Recently, a new IGH isotype specific to teleosts had been identified from zebra fish, rainbow trout, and fugu. In zebra fish, the domains of this new gene are present upstream of the μ region along the IGH locus. During this study, a novel IGH chimera (IgM-IgZ) has been discovered from common carp. The cloned cDNA encodes a typical leader peptide, a variable region, two constant regions, and a secretory tail. The first constant region is made up of the CH1 domain of carp IgM, while the second constant region shares a high similarity to the C H4 domain of the IgZ from zebrafish. Southern hybridization studies of the μ and ζ domains, conducted separately, revealed the presence of at least three copies of the respective genes, and μ and ζ domains might be present on the same loci, although far apart. Expression studies of the IGH genes suggest that there is an increase in chimeric immunoglobulin gene transcription when stimulated with lipopolysaccharide.
AB - In fish, two types of immunoglobulin heavy chain (IGH) genes, namely, IgM and IgD, have been cloned and characterized. Recently, a new IGH isotype specific to teleosts had been identified from zebra fish, rainbow trout, and fugu. In zebra fish, the domains of this new gene are present upstream of the μ region along the IGH locus. During this study, a novel IGH chimera (IgM-IgZ) has been discovered from common carp. The cloned cDNA encodes a typical leader peptide, a variable region, two constant regions, and a secretory tail. The first constant region is made up of the CH1 domain of carp IgM, while the second constant region shares a high similarity to the C H4 domain of the IgZ from zebrafish. Southern hybridization studies of the μ and ζ domains, conducted separately, revealed the presence of at least three copies of the respective genes, and μ and ζ domains might be present on the same loci, although far apart. Expression studies of the IGH genes suggest that there is an increase in chimeric immunoglobulin gene transcription when stimulated with lipopolysaccharide.
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U2 - 10.1007/s00251-005-0015-z
DO - 10.1007/s00251-005-0015-z
M3 - Article
C2 - 16025325
AN - SCOPUS:25844519738
VL - 57
SP - 458
EP - 463
JO - Immunogenetics
JF - Immunogenetics
SN - 0093-7711
IS - 6
ER -