Discovery of highly reactive peptide tag by ELISA-type screening for specific cysteine conjugation

Nobutaka Kurashige, Hirokazu Fuchida, Shigekazu Tabata, Shohei Uchinomiya, Akio Ojida

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We report the discovery of a highly reactive peptide tag for the specific cysteine conjugation of proteins. Screening of cysteine-containing peptides using ELISA-type screening yielded a 19-amino acid tag (DCPPPDDAADDAADDAADD), named DCP3 tag, which enabled the rapid and selective labeling of the tag-fused protein with a synthetic zinc complex on the surface of living cells.

Original languageEnglish
Pages (from-to)3486-3489
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume27
Issue number15
DOIs
Publication statusPublished - 2017

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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