Discovery of highly reactive peptide tag by ELISA-type screening for specific cysteine conjugation

Nobutaka Kurashige, Hirokazu Fuchida, Shigekazu Tabata, Shohei Uchinomiya, Akio Ojida

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We report the discovery of a highly reactive peptide tag for the specific cysteine conjugation of proteins. Screening of cysteine-containing peptides using ELISA-type screening yielded a 19-amino acid tag (DCPPPDDAADDAADDAADD), named DCP3 tag, which enabled the rapid and selective labeling of the tag-fused protein with a synthetic zinc complex on the surface of living cells.

Original languageEnglish
JournalBioorganic and Medicinal Chemistry Letters
DOIs
Publication statusAccepted/In press - Apr 18 2017

Fingerprint

Cysteine
Screening
Enzyme-Linked Immunosorbent Assay
Peptides
Labeling
Zinc
Proteins
Cells
Amino Acids

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Discovery of highly reactive peptide tag by ELISA-type screening for specific cysteine conjugation. / Kurashige, Nobutaka; Fuchida, Hirokazu; Tabata, Shigekazu; Uchinomiya, Shohei; Ojida, Akio.

In: Bioorganic and Medicinal Chemistry Letters, 18.04.2017.

Research output: Contribution to journalArticle

@article{84d850f28415456a8cb37878bab75a76,
title = "Discovery of highly reactive peptide tag by ELISA-type screening for specific cysteine conjugation",
abstract = "We report the discovery of a highly reactive peptide tag for the specific cysteine conjugation of proteins. Screening of cysteine-containing peptides using ELISA-type screening yielded a 19-amino acid tag (DCPPPDDAADDAADDAADD), named DCP3 tag, which enabled the rapid and selective labeling of the tag-fused protein with a synthetic zinc complex on the surface of living cells.",
author = "Nobutaka Kurashige and Hirokazu Fuchida and Shigekazu Tabata and Shohei Uchinomiya and Akio Ojida",
year = "2017",
month = "4",
day = "18",
doi = "10.1016/j.bmcl.2017.05.069",
language = "English",
journal = "Bioorganic and Medicinal Chemistry Letters",
issn = "0960-894X",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Discovery of highly reactive peptide tag by ELISA-type screening for specific cysteine conjugation

AU - Kurashige, Nobutaka

AU - Fuchida, Hirokazu

AU - Tabata, Shigekazu

AU - Uchinomiya, Shohei

AU - Ojida, Akio

PY - 2017/4/18

Y1 - 2017/4/18

N2 - We report the discovery of a highly reactive peptide tag for the specific cysteine conjugation of proteins. Screening of cysteine-containing peptides using ELISA-type screening yielded a 19-amino acid tag (DCPPPDDAADDAADDAADD), named DCP3 tag, which enabled the rapid and selective labeling of the tag-fused protein with a synthetic zinc complex on the surface of living cells.

AB - We report the discovery of a highly reactive peptide tag for the specific cysteine conjugation of proteins. Screening of cysteine-containing peptides using ELISA-type screening yielded a 19-amino acid tag (DCPPPDDAADDAADDAADD), named DCP3 tag, which enabled the rapid and selective labeling of the tag-fused protein with a synthetic zinc complex on the surface of living cells.

UR - http://www.scopus.com/inward/record.url?scp=85020056063&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020056063&partnerID=8YFLogxK

U2 - 10.1016/j.bmcl.2017.05.069

DO - 10.1016/j.bmcl.2017.05.069

M3 - Article

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

SN - 0960-894X

ER -