Discriminant and simultaneous HPLC analysis of reducing and nonreducing monosaccharides on a polyethyleneimine-attached hydrophilic interaction liquid chromatography column

Hsuan Chung, Akihiro Shimura, Toshiro Matsui

Research output: Contribution to journalArticle

Abstract

The aim of this study was to develop an analytical method for the discrimination of reducing and non-reducing monosaccharides including rare sugars on a prototype hydrophilic interaction liquid chromatography (HILIC) column. The HILIC column was composed of a glycidyl methacrylate-ethylene glycol dimethacrylate copolymer attached to 8 wt% polyethyleneimine. Seven monosaccharides (reducing aldoses: D-xylose, D-allose, and D-glucose; non-reducing ketoses: D-fructose, D-psicose, D-sorbose, and D-tagatose) were targeted. In individual HPLC analysis of both aldoses and ketoses, elution with 90 v/v% acetonitrile permitted successful detection and separation only for the non-reducing ketoses. Besides, elution of a mixture of ketoses and aldoses with 85 v/v% acetonitrile containing 5 mmol/L sodium 1-octanesulfonate (OS) (pH 4.8) enabled the simultaneous separation of all seven monosaccharides within 40 min. The polyethyleneimine-attached HILIC column allowed discriminant HPLC analysis for non-reducing ketoses using elution with 90 v/v% acetonitrile, whereas elution with 85 v/v% acetonitrile containing 5 mmol/L OS simultaneously detected both ketoses and aldoses.

Original languageEnglish
Pages (from-to)501-508
Number of pages8
JournalFood Science and Technology Research
Volume24
Issue number3
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Ketoses
ketoses
Polyethyleneimine
Monosaccharides
Liquid chromatography
monosaccharides
aldoses
Acetonitrile
Hydrophobic and Hydrophilic Interactions
Liquid Chromatography
high performance liquid chromatography
High Pressure Liquid Chromatography
Fructose
Xylose
allose
Discriminant analysis
psicose
Ethylene glycol
Sorbose
sorbose

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering
  • Marketing

Cite this

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abstract = "The aim of this study was to develop an analytical method for the discrimination of reducing and non-reducing monosaccharides including rare sugars on a prototype hydrophilic interaction liquid chromatography (HILIC) column. The HILIC column was composed of a glycidyl methacrylate-ethylene glycol dimethacrylate copolymer attached to 8 wt{\%} polyethyleneimine. Seven monosaccharides (reducing aldoses: D-xylose, D-allose, and D-glucose; non-reducing ketoses: D-fructose, D-psicose, D-sorbose, and D-tagatose) were targeted. In individual HPLC analysis of both aldoses and ketoses, elution with 90 v/v{\%} acetonitrile permitted successful detection and separation only for the non-reducing ketoses. Besides, elution of a mixture of ketoses and aldoses with 85 v/v{\%} acetonitrile containing 5 mmol/L sodium 1-octanesulfonate (OS) (pH 4.8) enabled the simultaneous separation of all seven monosaccharides within 40 min. The polyethyleneimine-attached HILIC column allowed discriminant HPLC analysis for non-reducing ketoses using elution with 90 v/v{\%} acetonitrile, whereas elution with 85 v/v{\%} acetonitrile containing 5 mmol/L OS simultaneously detected both ketoses and aldoses.",
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AB - The aim of this study was to develop an analytical method for the discrimination of reducing and non-reducing monosaccharides including rare sugars on a prototype hydrophilic interaction liquid chromatography (HILIC) column. The HILIC column was composed of a glycidyl methacrylate-ethylene glycol dimethacrylate copolymer attached to 8 wt% polyethyleneimine. Seven monosaccharides (reducing aldoses: D-xylose, D-allose, and D-glucose; non-reducing ketoses: D-fructose, D-psicose, D-sorbose, and D-tagatose) were targeted. In individual HPLC analysis of both aldoses and ketoses, elution with 90 v/v% acetonitrile permitted successful detection and separation only for the non-reducing ketoses. Besides, elution of a mixture of ketoses and aldoses with 85 v/v% acetonitrile containing 5 mmol/L sodium 1-octanesulfonate (OS) (pH 4.8) enabled the simultaneous separation of all seven monosaccharides within 40 min. The polyethyleneimine-attached HILIC column allowed discriminant HPLC analysis for non-reducing ketoses using elution with 90 v/v% acetonitrile, whereas elution with 85 v/v% acetonitrile containing 5 mmol/L OS simultaneously detected both ketoses and aldoses.

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