Discrimination between N- and C-termini of polypeptides by a two-dimensional array of helical poly(L-glutamic acid) rods on gold surfaces

Masazo Niwa, Masa-Aki Morikawa, Nobuyuki Higashi

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

A novel poly(L-glutamic acid) (PLGA) amphiphile, PLGA- SS, which has a disulfide bond at the N-terminus, formed spontaneously an adsorbed monolayer on gold substrates from aqueous solutions with various pHs. The adsorption rates of PLGA-SS were characterized by electrochemistry using a model of a microelectode array. When the monolayer was prepared at a lower pH, at which the PLGA segment takes the α-helix structure, a densely packed monolayer was formed, and in contrast, the preparation at higher pH was found to provide a less packed monolayer. Subsequently, the interaction of this monolayer with guest PLGAs, PLGA-Fc-N and -C, which have a ferrocenyl group at the N- and C-termini, respectively, was examined by means of the quartz crystal microbalance (QCM) technique. The frequency shift of QCM revealed that a stoichiometric interaction could be caused between the PLGA-SS monolayer and both guest PLGAs. The electron-transfer experiment and angle-dependent X-ray photoelectron spectroscopy were also applied to elucidate such a specific interaction. As a result, the host helix monolayer was found to capture the guest helix PLGAs through an antiparallel, side-by-side helix-macrodipole interaction.

Original languageEnglish
Pages (from-to)5088-5092
Number of pages5
JournalLangmuir
Volume15
Issue number15
DOIs
Publication statusPublished - Jul 20 1999
Externally publishedYes

Fingerprint

glutamic acid
Polypeptides
polypeptides
Gold
discrimination
Glutamic Acid
Monolayers
rods
gold
helices
Peptides
Acids
quartz crystals
Quartz crystal microbalances
microbalances
interactions
disulfides
Amphiphiles
electrochemistry
Electrochemistry

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Discrimination between N- and C-termini of polypeptides by a two-dimensional array of helical poly(L-glutamic acid) rods on gold surfaces. / Niwa, Masazo; Morikawa, Masa-Aki; Higashi, Nobuyuki.

In: Langmuir, Vol. 15, No. 15, 20.07.1999, p. 5088-5092.

Research output: Contribution to journalArticle

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