Abstract
A novel poly(L-glutamic acid) (PLGA) amphiphile, PLGA- SS, which has a disulfide bond at the N-terminus, formed spontaneously an adsorbed monolayer on gold substrates from aqueous solutions with various pHs. The adsorption rates of PLGA-SS were characterized by electrochemistry using a model of a microelectode array. When the monolayer was prepared at a lower pH, at which the PLGA segment takes the α-helix structure, a densely packed monolayer was formed, and in contrast, the preparation at higher pH was found to provide a less packed monolayer. Subsequently, the interaction of this monolayer with guest PLGAs, PLGA-Fc-N and -C, which have a ferrocenyl group at the N- and C-termini, respectively, was examined by means of the quartz crystal microbalance (QCM) technique. The frequency shift of QCM revealed that a stoichiometric interaction could be caused between the PLGA-SS monolayer and both guest PLGAs. The electron-transfer experiment and angle-dependent X-ray photoelectron spectroscopy were also applied to elucidate such a specific interaction. As a result, the host helix monolayer was found to capture the guest helix PLGAs through an antiparallel, side-by-side helix-macrodipole interaction.
| Original language | English |
|---|---|
| Pages (from-to) | 5088-5092 |
| Number of pages | 5 |
| Journal | Langmuir |
| Volume | 15 |
| Issue number | 15 |
| DOIs | |
| Publication status | Published - Jul 20 1999 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Condensed Matter Physics
- Surfaces and Interfaces
- Spectroscopy
- Electrochemistry
Cite this
Discrimination between N- and C-termini of polypeptides by a two-dimensional array of helical poly(L-glutamic acid) rods on gold surfaces. / Niwa, Masazo; Morikawa, Masa-Aki; Higashi, Nobuyuki.
In: Langmuir, Vol. 15, No. 15, 20.07.1999, p. 5088-5092.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Discrimination between N- and C-termini of polypeptides by a two-dimensional array of helical poly(L-glutamic acid) rods on gold surfaces
AU - Niwa, Masazo
AU - Morikawa, Masa-Aki
AU - Higashi, Nobuyuki
PY - 1999/7/20
Y1 - 1999/7/20
N2 - A novel poly(L-glutamic acid) (PLGA) amphiphile, PLGA- SS, which has a disulfide bond at the N-terminus, formed spontaneously an adsorbed monolayer on gold substrates from aqueous solutions with various pHs. The adsorption rates of PLGA-SS were characterized by electrochemistry using a model of a microelectode array. When the monolayer was prepared at a lower pH, at which the PLGA segment takes the α-helix structure, a densely packed monolayer was formed, and in contrast, the preparation at higher pH was found to provide a less packed monolayer. Subsequently, the interaction of this monolayer with guest PLGAs, PLGA-Fc-N and -C, which have a ferrocenyl group at the N- and C-termini, respectively, was examined by means of the quartz crystal microbalance (QCM) technique. The frequency shift of QCM revealed that a stoichiometric interaction could be caused between the PLGA-SS monolayer and both guest PLGAs. The electron-transfer experiment and angle-dependent X-ray photoelectron spectroscopy were also applied to elucidate such a specific interaction. As a result, the host helix monolayer was found to capture the guest helix PLGAs through an antiparallel, side-by-side helix-macrodipole interaction.
AB - A novel poly(L-glutamic acid) (PLGA) amphiphile, PLGA- SS, which has a disulfide bond at the N-terminus, formed spontaneously an adsorbed monolayer on gold substrates from aqueous solutions with various pHs. The adsorption rates of PLGA-SS were characterized by electrochemistry using a model of a microelectode array. When the monolayer was prepared at a lower pH, at which the PLGA segment takes the α-helix structure, a densely packed monolayer was formed, and in contrast, the preparation at higher pH was found to provide a less packed monolayer. Subsequently, the interaction of this monolayer with guest PLGAs, PLGA-Fc-N and -C, which have a ferrocenyl group at the N- and C-termini, respectively, was examined by means of the quartz crystal microbalance (QCM) technique. The frequency shift of QCM revealed that a stoichiometric interaction could be caused between the PLGA-SS monolayer and both guest PLGAs. The electron-transfer experiment and angle-dependent X-ray photoelectron spectroscopy were also applied to elucidate such a specific interaction. As a result, the host helix monolayer was found to capture the guest helix PLGAs through an antiparallel, side-by-side helix-macrodipole interaction.
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U2 - 10.1021/la9817258
DO - 10.1021/la9817258
M3 - Article
AN - SCOPUS:0344541715
VL - 15
SP - 5088
EP - 5092
JO - Langmuir
JF - Langmuir
SN - 0743-7463
IS - 15
ER -