Discrimination between stable and dynamic components of protein complexes by means of quantitative proteomics

Keiji Kito, Noriko Kawaguchi, Satoshi Okada, Takashi Ito

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

To discriminate between stable and dynamic protein-protein interactions, we propose a strategy in which cells with and without tagged bait are differentially labeled with stable isotope and combined prior to complex purification. Mass-spectrometric analysis of the purified complexes identifies stable and dynamic components as those derived exclusively from the tagged cells and those from both cells, respectively. We successfully applied this strategy to analyze two yeast protein complexes, eIF2B-eIF2 and cyclin-Cdc28.

Original languageEnglish
Pages (from-to)2366-2370
Number of pages5
JournalProteomics
Volume8
Issue number12
DOIs
Publication statusPublished - Jun 1 2008
Externally publishedYes

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Proteomics
Cyclins
Fungal Proteins
Isotopes
Purification
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Discrimination between stable and dynamic components of protein complexes by means of quantitative proteomics. / Kito, Keiji; Kawaguchi, Noriko; Okada, Satoshi; Ito, Takashi.

In: Proteomics, Vol. 8, No. 12, 01.06.2008, p. 2366-2370.

Research output: Contribution to journalArticle

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