Eight hundred and thirty-five sugi (Cryptomerica japonica D. Don) plus-tree clones from the Kanto Breeding Region were genotyped using three highly polymorphic SSR markers. With a mean observed heterozygosity of 0.831 and discrimination power at a locus varying from 0.98 to 0.99, the SSR markers were more effective for discrimination than RAPD (Random Amplified Polymorphic DNA) markers. Although five other markers were added to discriminate the remaining 189 sugi plus-tree that indicated the same genotype using three markers, only 6 plus-tree clones possessed the original genotype. One of 8 SSR markers, Cjgssr149, seemed to be multi-locus, and the SSR motifs of two clones were sequenced to verified whether it was multi-locus or not. As a result, it revealed that a duplicated SSR region was amplified.
|Number of pages||4|
|Journal||Nihon Ringakkai Shi/Journal of the Japanese Forestry Society|
|Publication status||Published - 2006|
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