Discrimination of sugi (Cryptomeria japonica D. Don) plus-tree clones using SSR marker analysis

T. Hirao, Atsushi Watanabe, Y. Goto-Fukuda, T. Kondo, K. Takata

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Eight hundred and thirty-five sugi (Cryptomerica japonica D. Don) plus-tree clones from the Kanto Breeding Region were genotyped using three highly polymorphic SSR markers. With a mean observed heterozygosity of 0.831 and discrimination power at a locus varying from 0.98 to 0.99, the SSR markers were more effective for discrimination than RAPD (Random Amplified Polymorphic DNA) markers. Although five other markers were added to discriminate the remaining 189 sugi plus-tree that indicated the same genotype using three markers, only 6 plus-tree clones possessed the original genotype. One of 8 SSR markers, Cjgssr149, seemed to be multi-locus, and the SSR motifs of two clones were sequenced to verified whether it was multi-locus or not. As a result, it revealed that a duplicated SSR region was amplified.

Original languageEnglish
Pages (from-to)202-205
Number of pages4
JournalNihon Ringakkai Shi/Journal of the Japanese Forestry Society
Volume88
Issue number3
Publication statusPublished - 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)

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