Discrimination of taste qualities among mouse fungiform taste bud cells

Ryusuke Yoshida, Aya Miyauchi, Toshiaki Yasuo, Masafumi Jyotaki, Yoshihiro Murata, Keiko Nakano, Noriatsu Shigemura, Yuchio Yanagawa, Kunihiko Obata, Hiroshi Ueno, Robert F. Margolskee, Yuzo Ninomiya

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Multiple lines of evidence from molecular studies indicate that individual taste qualities are encoded by distinct taste receptor cells. In contrast, many physiological studies have found that a significant proportion of taste cells respond to multiple taste qualities. To reconcile this apparent discrepancy and to identify taste cells that underlie each taste quality, we investigated taste responses of individual mouse fungiform taste cells that express gustducin or GAD67, markers for specific types of taste cells. Type II taste cells respond to sweet, bitter or umami tastants, express taste receptors, gustducin and other transduction components. Type III cells possess putative sour taste receptors, and have well elaborated conventional synapses. Consistent with these findings we found that gustducin-expressing Type II taste cells responded best to sweet (25/49), bitter (20/49) or umami (4/49) stimuli, while all GAD67 (Type III) taste cells examined (44/44) responded to sour stimuli and a portion of them showed multiple taste sensitivities, suggesting discrimination of each taste quality among taste bud cells. These results were largely consistent with those previously reported with circumvallate papillae taste cells. Bitter-best taste cells responded to multiple bitter compounds such as quinine, denatonium and cyclohexamide. Three sour compounds, HCl, acetic acid and citric acid, elicited responses in sour-best taste cells. These results suggest that taste cells may be capable of recognizing multiple taste compounds that elicit similar taste sensation. We did not find any NaCl-best cells among the gustducin and GAD67 taste cells, raising the possibility that salt sensitive taste cells comprise a different population.

Original languageEnglish
Pages (from-to)4425-4439
Number of pages15
JournalJournal of Physiology
Volume587
Issue number18
DOIs
Publication statusPublished - Sep 1 2009

Fingerprint

Taste Buds

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

Yoshida, R., Miyauchi, A., Yasuo, T., Jyotaki, M., Murata, Y., Nakano, K., ... Ninomiya, Y. (2009). Discrimination of taste qualities among mouse fungiform taste bud cells. Journal of Physiology, 587(18), 4425-4439. https://doi.org/10.1113/jphysiol.2009.175075

Discrimination of taste qualities among mouse fungiform taste bud cells. / Yoshida, Ryusuke; Miyauchi, Aya; Yasuo, Toshiaki; Jyotaki, Masafumi; Murata, Yoshihiro; Nakano, Keiko; Shigemura, Noriatsu; Yanagawa, Yuchio; Obata, Kunihiko; Ueno, Hiroshi; Margolskee, Robert F.; Ninomiya, Yuzo.

In: Journal of Physiology, Vol. 587, No. 18, 01.09.2009, p. 4425-4439.

Research output: Contribution to journalArticle

Yoshida, R, Miyauchi, A, Yasuo, T, Jyotaki, M, Murata, Y, Nakano, K, Shigemura, N, Yanagawa, Y, Obata, K, Ueno, H, Margolskee, RF & Ninomiya, Y 2009, 'Discrimination of taste qualities among mouse fungiform taste bud cells', Journal of Physiology, vol. 587, no. 18, pp. 4425-4439. https://doi.org/10.1113/jphysiol.2009.175075
Yoshida R, Miyauchi A, Yasuo T, Jyotaki M, Murata Y, Nakano K et al. Discrimination of taste qualities among mouse fungiform taste bud cells. Journal of Physiology. 2009 Sep 1;587(18):4425-4439. https://doi.org/10.1113/jphysiol.2009.175075
Yoshida, Ryusuke ; Miyauchi, Aya ; Yasuo, Toshiaki ; Jyotaki, Masafumi ; Murata, Yoshihiro ; Nakano, Keiko ; Shigemura, Noriatsu ; Yanagawa, Yuchio ; Obata, Kunihiko ; Ueno, Hiroshi ; Margolskee, Robert F. ; Ninomiya, Yuzo. / Discrimination of taste qualities among mouse fungiform taste bud cells. In: Journal of Physiology. 2009 ; Vol. 587, No. 18. pp. 4425-4439.
@article{c05863d41c1e43018ab4a19f3dfd8f33,
title = "Discrimination of taste qualities among mouse fungiform taste bud cells",
abstract = "Multiple lines of evidence from molecular studies indicate that individual taste qualities are encoded by distinct taste receptor cells. In contrast, many physiological studies have found that a significant proportion of taste cells respond to multiple taste qualities. To reconcile this apparent discrepancy and to identify taste cells that underlie each taste quality, we investigated taste responses of individual mouse fungiform taste cells that express gustducin or GAD67, markers for specific types of taste cells. Type II taste cells respond to sweet, bitter or umami tastants, express taste receptors, gustducin and other transduction components. Type III cells possess putative sour taste receptors, and have well elaborated conventional synapses. Consistent with these findings we found that gustducin-expressing Type II taste cells responded best to sweet (25/49), bitter (20/49) or umami (4/49) stimuli, while all GAD67 (Type III) taste cells examined (44/44) responded to sour stimuli and a portion of them showed multiple taste sensitivities, suggesting discrimination of each taste quality among taste bud cells. These results were largely consistent with those previously reported with circumvallate papillae taste cells. Bitter-best taste cells responded to multiple bitter compounds such as quinine, denatonium and cyclohexamide. Three sour compounds, HCl, acetic acid and citric acid, elicited responses in sour-best taste cells. These results suggest that taste cells may be capable of recognizing multiple taste compounds that elicit similar taste sensation. We did not find any NaCl-best cells among the gustducin and GAD67 taste cells, raising the possibility that salt sensitive taste cells comprise a different population.",
author = "Ryusuke Yoshida and Aya Miyauchi and Toshiaki Yasuo and Masafumi Jyotaki and Yoshihiro Murata and Keiko Nakano and Noriatsu Shigemura and Yuchio Yanagawa and Kunihiko Obata and Hiroshi Ueno and Margolskee, {Robert F.} and Yuzo Ninomiya",
year = "2009",
month = "9",
day = "1",
doi = "10.1113/jphysiol.2009.175075",
language = "English",
volume = "587",
pages = "4425--4439",
journal = "Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "18",

}

TY - JOUR

T1 - Discrimination of taste qualities among mouse fungiform taste bud cells

AU - Yoshida, Ryusuke

AU - Miyauchi, Aya

AU - Yasuo, Toshiaki

AU - Jyotaki, Masafumi

AU - Murata, Yoshihiro

AU - Nakano, Keiko

AU - Shigemura, Noriatsu

AU - Yanagawa, Yuchio

AU - Obata, Kunihiko

AU - Ueno, Hiroshi

AU - Margolskee, Robert F.

AU - Ninomiya, Yuzo

PY - 2009/9/1

Y1 - 2009/9/1

N2 - Multiple lines of evidence from molecular studies indicate that individual taste qualities are encoded by distinct taste receptor cells. In contrast, many physiological studies have found that a significant proportion of taste cells respond to multiple taste qualities. To reconcile this apparent discrepancy and to identify taste cells that underlie each taste quality, we investigated taste responses of individual mouse fungiform taste cells that express gustducin or GAD67, markers for specific types of taste cells. Type II taste cells respond to sweet, bitter or umami tastants, express taste receptors, gustducin and other transduction components. Type III cells possess putative sour taste receptors, and have well elaborated conventional synapses. Consistent with these findings we found that gustducin-expressing Type II taste cells responded best to sweet (25/49), bitter (20/49) or umami (4/49) stimuli, while all GAD67 (Type III) taste cells examined (44/44) responded to sour stimuli and a portion of them showed multiple taste sensitivities, suggesting discrimination of each taste quality among taste bud cells. These results were largely consistent with those previously reported with circumvallate papillae taste cells. Bitter-best taste cells responded to multiple bitter compounds such as quinine, denatonium and cyclohexamide. Three sour compounds, HCl, acetic acid and citric acid, elicited responses in sour-best taste cells. These results suggest that taste cells may be capable of recognizing multiple taste compounds that elicit similar taste sensation. We did not find any NaCl-best cells among the gustducin and GAD67 taste cells, raising the possibility that salt sensitive taste cells comprise a different population.

AB - Multiple lines of evidence from molecular studies indicate that individual taste qualities are encoded by distinct taste receptor cells. In contrast, many physiological studies have found that a significant proportion of taste cells respond to multiple taste qualities. To reconcile this apparent discrepancy and to identify taste cells that underlie each taste quality, we investigated taste responses of individual mouse fungiform taste cells that express gustducin or GAD67, markers for specific types of taste cells. Type II taste cells respond to sweet, bitter or umami tastants, express taste receptors, gustducin and other transduction components. Type III cells possess putative sour taste receptors, and have well elaborated conventional synapses. Consistent with these findings we found that gustducin-expressing Type II taste cells responded best to sweet (25/49), bitter (20/49) or umami (4/49) stimuli, while all GAD67 (Type III) taste cells examined (44/44) responded to sour stimuli and a portion of them showed multiple taste sensitivities, suggesting discrimination of each taste quality among taste bud cells. These results were largely consistent with those previously reported with circumvallate papillae taste cells. Bitter-best taste cells responded to multiple bitter compounds such as quinine, denatonium and cyclohexamide. Three sour compounds, HCl, acetic acid and citric acid, elicited responses in sour-best taste cells. These results suggest that taste cells may be capable of recognizing multiple taste compounds that elicit similar taste sensation. We did not find any NaCl-best cells among the gustducin and GAD67 taste cells, raising the possibility that salt sensitive taste cells comprise a different population.

UR - http://www.scopus.com/inward/record.url?scp=70450191418&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70450191418&partnerID=8YFLogxK

U2 - 10.1113/jphysiol.2009.175075

DO - 10.1113/jphysiol.2009.175075

M3 - Article

C2 - 19622604

AN - SCOPUS:70450191418

VL - 587

SP - 4425

EP - 4439

JO - Journal of Physiology

JF - Journal of Physiology

SN - 0022-3751

IS - 18

ER -