Discrimination of teak (Tectona grandis) plus trees using selected random amplified polymorphic DNA (RAPD) markers

Atsushi Watanabe, A. Widyatmoko, A. Rimbawanto, S. Shiraishi

Research output: Contribution to journalReview article

4 Citations (Scopus)

Abstract

To achieve a highly reliable clone management of teak (Tectona grandis) plus trees, useful DNA molecular markers were surveyed using RAPD analysis and their ability to discriminate among plus tree clones was examined. A primary screening was performed for 120 arbitrary decamer primers, and 24 primers that generated 26 clear and unambiguous fragments were selected. In the secondary screening, the reproducibility of each fragment was investigated by six repetitions of polymerase chain reaction, and 13 fragments found to be most reproducible were finally selected. Evaluation of the discriminatory powers of these fragments suggested that the selected RAPD markers would be useful in the clone management of teak plus trees.

Original languageEnglish
Pages (from-to)17-24
Number of pages8
JournalJournal of Tropical Forest Science
Volume16
Issue number1
Publication statusPublished - Jan 2004

Fingerprint

plus trees
random amplified polymorphic DNA technique
Tectona grandis
Genetic Markers
clone
Clone Cells
clones
DNA
genetic markers
screening
reproducibility
polymerase chain reaction
Polymerase Chain Reaction
marker

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Discrimination of teak (Tectona grandis) plus trees using selected random amplified polymorphic DNA (RAPD) markers. / Watanabe, Atsushi; Widyatmoko, A.; Rimbawanto, A.; Shiraishi, S.

In: Journal of Tropical Forest Science, Vol. 16, No. 1, 01.2004, p. 17-24.

Research output: Contribution to journalReview article

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