TY - JOUR
T1 - Disinfectant potential in inactivation of epidemic keratoconjunctivitis-related adenoviruses by potassium peroxymonosulfate
AU - Hashizume, Mei
AU - Aoki, Koki
AU - Ohno, Shigeaki
AU - Kitaichi, Nobuyoshi
AU - Yawata, Nobuyo
AU - Gonzalez, Gabriel
AU - Nonaka, Hirotaka
AU - Sato, Seiichi
AU - Takaoka, Akinori
N1 - Funding Information:
This study demonstrated that potassium peroxymonosulfate is a promising disinfectant for the prevention of epidemic keratoconjunctivitis nosocomial infections in ophthalmological clinics. Human adenovirus epidemic keratoconjunctivitis disinfectants prevention RUBYSTA Virkon edited-state corrected-proof typesetter ts1 We thank R. Hinokuma and S. Hashimoto for HAdV and H. Watanabe for helpful advice. Declaration of conflicting interests The author(s) declared no potential conflicts of interest with respect to the research, authorship and/or publication of this article. Funding The author(s) disclosed receipt of the following financial support for the research, authorship and/or publication of this article: This work was supported by financial support from KYORIN Medical Supply Co., Ltd (Tokyo, Japan). ORCID iD Akinori Takaoka https://orcid.org/0000-0002-6036-3757
Funding Information:
We thank R. Hinokuma and S. Hashimoto for HAdV and H. Watanabe for helpful advice. The author(s) disclosed receipt of the following financial support for the research, authorship and/or publication of this article: This work was supported by financial support from KYORIN Medical Supply Co., Ltd (Tokyo, Japan).
Publisher Copyright:
© The Author(s) 2019.
PY - 2021/3
Y1 - 2021/3
N2 - Purpose: The aim of this study was to test the antiviral effectivity of potassium peroxymonosulfate (RUBYSTA®, KYORIN) against five epidemic keratoconjunctivitis-related types of Human adenovirus D in vitro. Methods: Five types of Human adenovirus D (8, 37, 53, 54 and 56) were incubated with 1% potassium peroxymonosulfate, 0.1% sodium hypochlorite (NaClO) or alcohol-based disinfectant for 30 s or 1 min. These solutions were subjected to measurements of viral titres by infection assays in A549 cells. At day 6 post-infection, both, supernatants and cells, were collected and the viral genome was assessed by real-time polymerase chain reaction analysis. Results: Treatments with 1% potassium peroxymonosulfate led to significant reduction in all tested Human adenovirus D types comparable to disinfecting effects by 0.1% NaClO. Overall, potassium peroxymonosulfate demonstrated sufficient inactivation of the major epidemic keratoconjunctivitis-causing Human adenovirus D to be considered for disinfection and prevention purposes in ophthalmological clinics and hospitals. Conclusion: This study demonstrated that potassium peroxymonosulfate is a promising disinfectant for the prevention of epidemic keratoconjunctivitis nosocomial infections in ophthalmological clinics.
AB - Purpose: The aim of this study was to test the antiviral effectivity of potassium peroxymonosulfate (RUBYSTA®, KYORIN) against five epidemic keratoconjunctivitis-related types of Human adenovirus D in vitro. Methods: Five types of Human adenovirus D (8, 37, 53, 54 and 56) were incubated with 1% potassium peroxymonosulfate, 0.1% sodium hypochlorite (NaClO) or alcohol-based disinfectant for 30 s or 1 min. These solutions were subjected to measurements of viral titres by infection assays in A549 cells. At day 6 post-infection, both, supernatants and cells, were collected and the viral genome was assessed by real-time polymerase chain reaction analysis. Results: Treatments with 1% potassium peroxymonosulfate led to significant reduction in all tested Human adenovirus D types comparable to disinfecting effects by 0.1% NaClO. Overall, potassium peroxymonosulfate demonstrated sufficient inactivation of the major epidemic keratoconjunctivitis-causing Human adenovirus D to be considered for disinfection and prevention purposes in ophthalmological clinics and hospitals. Conclusion: This study demonstrated that potassium peroxymonosulfate is a promising disinfectant for the prevention of epidemic keratoconjunctivitis nosocomial infections in ophthalmological clinics.
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U2 - 10.1177/1120672119891408
DO - 10.1177/1120672119891408
M3 - Article
C2 - 31813297
AN - SCOPUS:85077376286
SN - 1120-6721
VL - 31
SP - 379
EP - 384
JO - European Journal of Ophthalmology
JF - European Journal of Ophthalmology
IS - 2
ER -