Disparity of MCP-1 mRNA and protein expressions between the carotid artery and the aorta in WHHL rabbits: One aspect involved in the regional difference in atherosclerosis

Eriko Tanaka, Hiroaki Shimokawa, Hitoshi Kamiuneten, Yasuhiro Eto, Yasuharu Matsumoto, Kunio Morishige, George Koike, Masaru Yoshinaga, Kensuke Egashira, Osamu Tokunaga, Masashi Shiomi, Akira Takeshita

    Research output: Contribution to journalArticlepeer-review

    21 Citations (Scopus)

    Abstract

    Objective - This study was designed to examine why in WHHL rabbits, muscular arteries, such as the carotid artery, are relatively resistant to atherosclerosis compared with the aorta, with a special reference to monocyte chemoattractant protein (MCP)-1. Methods and Results - MCP-1 mRNA expression was quantitated by Northern blot analysis, and its protein expression was quantitated by immunostaining and ELISA at the age of 1, 3, 6, and 12 months (n=5 to 6 each). In the aorta, atherosclerotic lesions were progressively developed with aging, and MCP-1 was highly expressed in endothelial cells and infiltrating macrophages. By contrast, in the carotid artery, atherosclerotic lesions and MCP-1 immunoreactivity were not evident throughout the experimental period. Unexpectedly, however, the extent of MCP-1 mRNA expression was comparable between the aorta and the carotid artery throughout the experimental period. Endothelial cells in primary culture from the aorta and the carotid artery expressed the same extent of MCP-1 mRNA on stimulation by oxidized LDL. There was no abnormality in primary structure of MCP-1 cDNA in WHHL. Conclusions - These results suggest that in WHHL, the atherosclerosis process, including MCP-1 protein expression, may be reduced in the carotid artery (and possibly in other muscular arteries), accounting in part for the regional resistance to atherosclerosis.

    Original languageEnglish
    Pages (from-to)244-250
    Number of pages7
    JournalArteriosclerosis, thrombosis, and vascular biology
    Volume23
    Issue number2
    DOIs
    Publication statusPublished - Feb 1 2003

    All Science Journal Classification (ASJC) codes

    • Cardiology and Cardiovascular Medicine

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