Displacement immunosensor based on surface plasmon resonance for rapid and highly sensitive detection of 2,4,6-trinitrotoluene

Takeshi Onodera, Yutaka Mizuta, Kazuhiko Horikawa, Praveen Singh, Kiyoshi Matsumoto, Norio Miura, Kiyoshi Toko

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

By making use of the antigen-antibody interaction and a surface plasmon resonance (SPR) sensor, we developed a measurement procedure of displacement immunoassay to rapidly detect 2,4,6-trinitrotoluene (TNT). In this procedure, TNT solutions were injected in 30 s at the end of flowing an anti-TNT antibody. Three kinds of sensor surfaces were modified with TNT analogues, namely, TNP-glycine, DNP-glycine, and DNP-acetic acid in a self-assembled monolayer containing ethylene glycol. We investigated which of the TNT analogues provided higher sensitivity using the displacement immunosensor. As a result, the limit of detection (LOD) of TNT was 0.4 ng/mL (ppb) when using the DNP-glycine-modified Au sensor surface with a one-minute flow of TNT solution. We concluded that the final TNT LOD was 0.9 ppb on the basis of experiments using the three different DNP-glycine-modified sensor surfaces. The LOD was 0.7 ppb when using the sensorgram slope 10 s after TNT injection. The displacement immunosensor can detect TNT at sub-ppb levels in 12 s.

Original languageEnglish
Pages (from-to)39-52
Number of pages14
JournalSensors and Materials
Volume23
Issue number1
Publication statusPublished - 2011

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Materials Science(all)

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