Displacement immunosensor based on surface plasmon resonance for rapid and highly sensitive detection of 2,4,6-trinitrotoluene

Takeshi Onodera, Yutaka Mizuta, Kazuhiko Horikawa, Praveen Singh, Kiyoshi Matsumoto, Norio Miura, Kiyoshi Toko

Research output: Contribution to journalArticle

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Abstract

By making use of the antigen-antibody interaction and a surface plasmon resonance (SPR) sensor, we developed a measurement procedure of displacement immunoassay to rapidly detect 2,4,6-trinitrotoluene (TNT). In this procedure, TNT solutions were injected in 30 s at the end of flowing an anti-TNT antibody. Three kinds of sensor surfaces were modified with TNT analogues, namely, TNP-glycine, DNP-glycine, and DNP-acetic acid in a self-assembled monolayer containing ethylene glycol. We investigated which of the TNT analogues provided higher sensitivity using the displacement immunosensor. As a result, the limit of detection (LOD) of TNT was 0.4 ng/mL (ppb) when using the DNP-glycine-modified Au sensor surface with a one-minute flow of TNT solution. We concluded that the final TNT LOD was 0.9 ppb on the basis of experiments using the three different DNP-glycine-modified sensor surfaces. The LOD was 0.7 ppb when using the sensorgram slope 10 s after TNT injection. The displacement immunosensor can detect TNT at sub-ppb levels in 12 s.

Original languageEnglish
Pages (from-to)39-52
Number of pages14
JournalSensors and Materials
Volume23
Issue number1
Publication statusPublished - 2011

Fingerprint

Trinitrotoluene
Immunosensors
trinitrotoluene
Surface plasmon resonance
surface plasmon resonance
Amino acids
Sensors
Antibodies
glycine
Glycine
Self assembled monolayers
Antigens
Ethylene glycol
Acetic acid
sensors
antibodies
analogs
immunoassay
Ethylene Glycol
antigens

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Materials Science(all)

Cite this

Displacement immunosensor based on surface plasmon resonance for rapid and highly sensitive detection of 2,4,6-trinitrotoluene. / Onodera, Takeshi; Mizuta, Yutaka; Horikawa, Kazuhiko; Singh, Praveen; Matsumoto, Kiyoshi; Miura, Norio; Toko, Kiyoshi.

In: Sensors and Materials, Vol. 23, No. 1, 2011, p. 39-52.

Research output: Contribution to journalArticle

Onodera, T, Mizuta, Y, Horikawa, K, Singh, P, Matsumoto, K, Miura, N & Toko, K 2011, 'Displacement immunosensor based on surface plasmon resonance for rapid and highly sensitive detection of 2,4,6-trinitrotoluene', Sensors and Materials, vol. 23, no. 1, pp. 39-52.
Onodera, Takeshi ; Mizuta, Yutaka ; Horikawa, Kazuhiko ; Singh, Praveen ; Matsumoto, Kiyoshi ; Miura, Norio ; Toko, Kiyoshi. / Displacement immunosensor based on surface plasmon resonance for rapid and highly sensitive detection of 2,4,6-trinitrotoluene. In: Sensors and Materials. 2011 ; Vol. 23, No. 1. pp. 39-52.
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