TY - JOUR
T1 - Disruption of clh-1, a chloride channel gene, results in a wider body of Caenorhabditis elegans
AU - Petalcorin, Mark I.R.
AU - Oka, Toshihiko
AU - Koga, Makoto
AU - Ogura, Ken Ichi
AU - Wada, Yoh
AU - Ohshima, Yasumi
AU - Futai, Masamitsu
PY - 1999/11/26
Y1 - 1999/11/26
N2 - We cloned the clh-1 gene coding for a putative ClC chloride channel in Caenorhabditis elegans. The gene product exhibited a high degree of homology with human ClC-1 and ClC-2. The clh-1 gene was predominantly expressed in the hypodermis, including seam cells. Null mutations of clh-1 caused a significantly wider body and an abnormal alae structure. High osmolarity in the culture medium restored the normal body width of the clh-1 mutants. These results suggest that the clh-1 gene contributes to maintenance of the body width through regulation of osmolarity.
AB - We cloned the clh-1 gene coding for a putative ClC chloride channel in Caenorhabditis elegans. The gene product exhibited a high degree of homology with human ClC-1 and ClC-2. The clh-1 gene was predominantly expressed in the hypodermis, including seam cells. Null mutations of clh-1 caused a significantly wider body and an abnormal alae structure. High osmolarity in the culture medium restored the normal body width of the clh-1 mutants. These results suggest that the clh-1 gene contributes to maintenance of the body width through regulation of osmolarity.
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U2 - 10.1006/jmbi.1999.3241
DO - 10.1006/jmbi.1999.3241
M3 - Article
C2 - 10610763
AN - SCOPUS:0033607475
SN - 0022-2836
VL - 294
SP - 347
EP - 355
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 2
ER -