Disruption of mouse XAB2 gene involved in pre-mRNA splicing, transcription and transcription-coupled DNA repair results in preimplantation lethality

Rie Yonemasu, Mitsuyoshi Minami, Yoshimichi Nakatsu, Masayo Takeuchi, Isao Kuraoka, Yoichi Matsuda, Yujiro Higashi, Hisato Kondoh, Kiyoji Tanaka

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The XAB2 protein (XPA-binding protein 2) with 15 tetratricopeptide repeat motifs has been isolated by virtue of its ability to interact with xeroderma pigmentosum group A (XPA) protein in the yeast two-hybrid system. It has been shown that XAB2 interacted with Cockayne syndrome groups A and B (CSA and CSB) proteins and RNA polymerase II, which are known to be involved in transcription-coupled repair (TCR) and transcription, and that the antibodies against XAB2 protein inhibited the recovery of RNA synthesis after UV irradiation and normal RNA synthesis when microinjected into living fibroblasts. These results have indicated that XAB2 is involved in TCR and transcription. In this report, to elucidate the function of XAB2 in vivo, two types of mutations were introduced into the XAB2 gene in mice: a deletion of the region encompassing the promoter and exons 1-4, and a deletion of the C-terminal 162 amino acids. Both types of XAB2-heterozygous mice appeared normal physiologically and behaviorally. However, XAB2-homozygotes were selectively absent among the newborn mice. A detailed analysis of embryos at different stages of development indicated that the XAB2-homozygous mutants could survive until the morula stage, but could not develop to the blastocyst stage. These results indicate that XAB2 has an essential function in mouse embryogenesis.

Original languageEnglish
Pages (from-to)479-491
Number of pages13
JournalDNA Repair
Volume4
Issue number4
DOIs
Publication statusPublished - Apr 4 2005
Externally publishedYes

Fingerprint

RNA Precursors
Transcription
DNA Repair
Xeroderma Pigmentosum Group A Protein
Repair
Genes
Cockayne Syndrome
DNA
RNA
Morula
Two-Hybrid System Techniques
RNA Polymerase II
Homozygote
Blastocyst
Fibroblasts
Hybrid systems
Genetic Promoter Regions
Yeast
Embryonic Development
Exons

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Disruption of mouse XAB2 gene involved in pre-mRNA splicing, transcription and transcription-coupled DNA repair results in preimplantation lethality. / Yonemasu, Rie; Minami, Mitsuyoshi; Nakatsu, Yoshimichi; Takeuchi, Masayo; Kuraoka, Isao; Matsuda, Yoichi; Higashi, Yujiro; Kondoh, Hisato; Tanaka, Kiyoji.

In: DNA Repair, Vol. 4, No. 4, 04.04.2005, p. 479-491.

Research output: Contribution to journalArticle

Yonemasu, Rie ; Minami, Mitsuyoshi ; Nakatsu, Yoshimichi ; Takeuchi, Masayo ; Kuraoka, Isao ; Matsuda, Yoichi ; Higashi, Yujiro ; Kondoh, Hisato ; Tanaka, Kiyoji. / Disruption of mouse XAB2 gene involved in pre-mRNA splicing, transcription and transcription-coupled DNA repair results in preimplantation lethality. In: DNA Repair. 2005 ; Vol. 4, No. 4. pp. 479-491.
@article{546c78a99a1044a2a90e8edd44f3eb4c,
title = "Disruption of mouse XAB2 gene involved in pre-mRNA splicing, transcription and transcription-coupled DNA repair results in preimplantation lethality",
abstract = "The XAB2 protein (XPA-binding protein 2) with 15 tetratricopeptide repeat motifs has been isolated by virtue of its ability to interact with xeroderma pigmentosum group A (XPA) protein in the yeast two-hybrid system. It has been shown that XAB2 interacted with Cockayne syndrome groups A and B (CSA and CSB) proteins and RNA polymerase II, which are known to be involved in transcription-coupled repair (TCR) and transcription, and that the antibodies against XAB2 protein inhibited the recovery of RNA synthesis after UV irradiation and normal RNA synthesis when microinjected into living fibroblasts. These results have indicated that XAB2 is involved in TCR and transcription. In this report, to elucidate the function of XAB2 in vivo, two types of mutations were introduced into the XAB2 gene in mice: a deletion of the region encompassing the promoter and exons 1-4, and a deletion of the C-terminal 162 amino acids. Both types of XAB2-heterozygous mice appeared normal physiologically and behaviorally. However, XAB2-homozygotes were selectively absent among the newborn mice. A detailed analysis of embryos at different stages of development indicated that the XAB2-homozygous mutants could survive until the morula stage, but could not develop to the blastocyst stage. These results indicate that XAB2 has an essential function in mouse embryogenesis.",
author = "Rie Yonemasu and Mitsuyoshi Minami and Yoshimichi Nakatsu and Masayo Takeuchi and Isao Kuraoka and Yoichi Matsuda and Yujiro Higashi and Hisato Kondoh and Kiyoji Tanaka",
year = "2005",
month = "4",
day = "4",
doi = "10.1016/j.dnarep.2004.12.004",
language = "English",
volume = "4",
pages = "479--491",
journal = "DNA Repair",
issn = "1568-7864",
publisher = "Elsevier",
number = "4",

}

TY - JOUR

T1 - Disruption of mouse XAB2 gene involved in pre-mRNA splicing, transcription and transcription-coupled DNA repair results in preimplantation lethality

AU - Yonemasu, Rie

AU - Minami, Mitsuyoshi

AU - Nakatsu, Yoshimichi

AU - Takeuchi, Masayo

AU - Kuraoka, Isao

AU - Matsuda, Yoichi

AU - Higashi, Yujiro

AU - Kondoh, Hisato

AU - Tanaka, Kiyoji

PY - 2005/4/4

Y1 - 2005/4/4

N2 - The XAB2 protein (XPA-binding protein 2) with 15 tetratricopeptide repeat motifs has been isolated by virtue of its ability to interact with xeroderma pigmentosum group A (XPA) protein in the yeast two-hybrid system. It has been shown that XAB2 interacted with Cockayne syndrome groups A and B (CSA and CSB) proteins and RNA polymerase II, which are known to be involved in transcription-coupled repair (TCR) and transcription, and that the antibodies against XAB2 protein inhibited the recovery of RNA synthesis after UV irradiation and normal RNA synthesis when microinjected into living fibroblasts. These results have indicated that XAB2 is involved in TCR and transcription. In this report, to elucidate the function of XAB2 in vivo, two types of mutations were introduced into the XAB2 gene in mice: a deletion of the region encompassing the promoter and exons 1-4, and a deletion of the C-terminal 162 amino acids. Both types of XAB2-heterozygous mice appeared normal physiologically and behaviorally. However, XAB2-homozygotes were selectively absent among the newborn mice. A detailed analysis of embryos at different stages of development indicated that the XAB2-homozygous mutants could survive until the morula stage, but could not develop to the blastocyst stage. These results indicate that XAB2 has an essential function in mouse embryogenesis.

AB - The XAB2 protein (XPA-binding protein 2) with 15 tetratricopeptide repeat motifs has been isolated by virtue of its ability to interact with xeroderma pigmentosum group A (XPA) protein in the yeast two-hybrid system. It has been shown that XAB2 interacted with Cockayne syndrome groups A and B (CSA and CSB) proteins and RNA polymerase II, which are known to be involved in transcription-coupled repair (TCR) and transcription, and that the antibodies against XAB2 protein inhibited the recovery of RNA synthesis after UV irradiation and normal RNA synthesis when microinjected into living fibroblasts. These results have indicated that XAB2 is involved in TCR and transcription. In this report, to elucidate the function of XAB2 in vivo, two types of mutations were introduced into the XAB2 gene in mice: a deletion of the region encompassing the promoter and exons 1-4, and a deletion of the C-terminal 162 amino acids. Both types of XAB2-heterozygous mice appeared normal physiologically and behaviorally. However, XAB2-homozygotes were selectively absent among the newborn mice. A detailed analysis of embryos at different stages of development indicated that the XAB2-homozygous mutants could survive until the morula stage, but could not develop to the blastocyst stage. These results indicate that XAB2 has an essential function in mouse embryogenesis.

UR - http://www.scopus.com/inward/record.url?scp=13844321120&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13844321120&partnerID=8YFLogxK

U2 - 10.1016/j.dnarep.2004.12.004

DO - 10.1016/j.dnarep.2004.12.004

M3 - Article

C2 - 15725628

AN - SCOPUS:13844321120

VL - 4

SP - 479

EP - 491

JO - DNA Repair

JF - DNA Repair

SN - 1568-7864

IS - 4

ER -