The Escherichia coil hslVU genes encode subunits of an ATP-dependent protease, a homolog of the eukaryotic 265 proteasome. We found that the hslU gene is required for the temperature (40°C)-sensitive phenotype of the dnaA46 mutant, while other soluble ATP-dependent proteases, La (Lon) and Ti (Clp), were unrelated to this dnaA46 phenotype. Disruption of the hslU gene inhibited cell growth at high temperatures. These observations suggest a specific in vivo role for HslVU protease in denatured proteins. As the absence of HslU produces minicells in M9 medium, the protease may be involved in the cell cycle regulation.
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - Dec 4 1996|
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology