Dissection of measles virus V protein in relation to its ability to block alpha/beta interferon signal transduction

Shinji Ohno, Nobuyuki Ono, Makoto Takeda, Kaoru Takeuchi, Yusuke Yanagi

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

Interferon (IFN)-α and -β are the main cytokines for innate immune responses against viral infections. To replicate efficiently in the hosts, viruses have evolved various countermeasures to the IFN response. The V protein of measles virus (MV) has been shown to block IFN-α/β signalling. Here, the wild-type JC-B strain of MV was shown to grow comparably in the presence and absence of IFN-α, whereas replication of the Edmonston tag strain recovered from cloned DNA was strongly suppressed in its presence. The V protein of the IC-B strain, but not the Edmonston tag strain, blocked IFN-α signalling. The V protein of the Edmonston strain from the ATCC also inhibited IFN-α signalling. There were three amino acid differences between the V proteins of the Edmonston ATCC and tag strains, and substitutions of both residues at positions 110 and 272 were required for the Edmonston ATCC V protein to lose IFN-antagonist activity. The P protein of the IC-B strain, which shares the N-terminal 231 aa residues with the V protein, also inhibited IFN-α signalling. Indeed, fragments comprising only those 231 residues of the IC-B and Edmonston ATCC V proteins, but not the Edmonston tag V protein, were able to block IFN-α signalling. However, the N-terminal region of the Edmonston tag V protein, when attached to the C-terminal region of the Edmonston ATCC V protein, inhibited IFN-α signalling. Taken together, our results indicate that both the N- and C-terminal regions contribute to the IFN-antagonist activity of the MV V protein.

Original languageEnglish
Pages (from-to)2991-2999
Number of pages9
JournalJournal of General Virology
Volume85
Issue number10
DOIs
Publication statusPublished - Oct 1 2004

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Interferon-beta
Interferon-alpha
Interferons
Dissection
Signal Transduction
Proteins
measles virus V protein
Measles virus
Virus Diseases
Innate Immunity
Cytokines
Viruses
Amino Acids

All Science Journal Classification (ASJC) codes

  • Virology

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Dissection of measles virus V protein in relation to its ability to block alpha/beta interferon signal transduction. / Ohno, Shinji; Ono, Nobuyuki; Takeda, Makoto; Takeuchi, Kaoru; Yanagi, Yusuke.

In: Journal of General Virology, Vol. 85, No. 10, 01.10.2004, p. 2991-2999.

Research output: Contribution to journalArticle

Ohno, Shinji ; Ono, Nobuyuki ; Takeda, Makoto ; Takeuchi, Kaoru ; Yanagi, Yusuke. / Dissection of measles virus V protein in relation to its ability to block alpha/beta interferon signal transduction. In: Journal of General Virology. 2004 ; Vol. 85, No. 10. pp. 2991-2999.
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