Distinct cytokine and T helper cell profiles between patients with multiple sclerosis who had or had not received interferon-beta

Hikaru Doi, Zi Ye Song, Satoshi Yoshimura, Takahisa Tateishi, Tomomi Yonekawa, Ryo Yamasaki, Hiroyuki Murai, Takuya Matsushita, Jun-Ichi Kira

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objectives Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system, and is generally considered to be mediated by T helper (Th) 1/Th17 cells. Interferon-β (IFNβ) is widely used as a disease-modifying MS drug, but its effects on Th17 cells are still disputed. Furthermore, the effects of IFNβ on Th9 cells have not been elucidated. The present study aimed to clarify the effects of IFNβ on cytokines/growth factors in cerebrospinal fluid (CSF) and cytokine-producing Th cells in peripheral blood.

Methods First, the frequency of IFNγ, interleukin (IL)-17A, IL-9, and IL-4-producing Th cells in peripheral blood lymphocytes was analyzed by flow cytometry in 34 MS patients and 15 healthy volunteers enrolled in the cytokine-producing Th cell study. Second, levels of 27 cytokines/growth factors in the CSF were measured using a multiple fluorescence bead-based immunoassay in 34 MS patients enrolled in the cytokines/growth factors study.

Results We found a significantly higher frequency of IL-4-IL-9+CD4+ T cells and lower frequency of IFNγ+IL-17A-CD4+ cells in peripheral blood lymphocytes in the 10 MS patients who had received IFNβ than in the 24 MS patients who had not received IFNβ or the 15 healthy controls (P < 0.05). The seven MS patients who received IFNβ showed significantly lower IL-17A levels in CSF than did the 27 MS patients who had not received IFNβ (P < 0.05).

Conclusions The present results suggest the suppression of IL-17A production in the central nervous system and augmentation of Th9 cells in the peripheral blood by IFNβ in MS patients.

Original languageEnglish
Pages (from-to)321-327
Number of pages7
JournalClinical and Experimental Neuroimmunology
Volume5
Issue number3
DOIs
Publication statusPublished - Jan 1 2014

Fingerprint

Interferon-beta
Helper-Inducer T-Lymphocytes
Interferons
Multiple Sclerosis
Cytokines
Interleukin-17
Interleukin-9
Cerebrospinal Fluid
Th17 Cells
Intercellular Signaling Peptides and Proteins
Interleukin-4
Central Nervous System
Lymphocytes
Th1 Cells
Demyelinating Diseases
Immunoassay
Healthy Volunteers
Flow Cytometry
Fluorescence
T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Neuroscience (miscellaneous)
  • Immunology
  • Immunology and Microbiology (miscellaneous)
  • Clinical Neurology

Cite this

Distinct cytokine and T helper cell profiles between patients with multiple sclerosis who had or had not received interferon-beta. / Doi, Hikaru; Song, Zi Ye; Yoshimura, Satoshi; Tateishi, Takahisa; Yonekawa, Tomomi; Yamasaki, Ryo; Murai, Hiroyuki; Matsushita, Takuya; Kira, Jun-Ichi.

In: Clinical and Experimental Neuroimmunology, Vol. 5, No. 3, 01.01.2014, p. 321-327.

Research output: Contribution to journalArticle

Doi, Hikaru ; Song, Zi Ye ; Yoshimura, Satoshi ; Tateishi, Takahisa ; Yonekawa, Tomomi ; Yamasaki, Ryo ; Murai, Hiroyuki ; Matsushita, Takuya ; Kira, Jun-Ichi. / Distinct cytokine and T helper cell profiles between patients with multiple sclerosis who had or had not received interferon-beta. In: Clinical and Experimental Neuroimmunology. 2014 ; Vol. 5, No. 3. pp. 321-327.
@article{d01173e57213495990a4d68161ba6237,
title = "Distinct cytokine and T helper cell profiles between patients with multiple sclerosis who had or had not received interferon-beta",
abstract = "Objectives Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system, and is generally considered to be mediated by T helper (Th) 1/Th17 cells. Interferon-β (IFNβ) is widely used as a disease-modifying MS drug, but its effects on Th17 cells are still disputed. Furthermore, the effects of IFNβ on Th9 cells have not been elucidated. The present study aimed to clarify the effects of IFNβ on cytokines/growth factors in cerebrospinal fluid (CSF) and cytokine-producing Th cells in peripheral blood.Methods First, the frequency of IFNγ, interleukin (IL)-17A, IL-9, and IL-4-producing Th cells in peripheral blood lymphocytes was analyzed by flow cytometry in 34 MS patients and 15 healthy volunteers enrolled in the cytokine-producing Th cell study. Second, levels of 27 cytokines/growth factors in the CSF were measured using a multiple fluorescence bead-based immunoassay in 34 MS patients enrolled in the cytokines/growth factors study.Results We found a significantly higher frequency of IL-4-IL-9+CD4+ T cells and lower frequency of IFNγ+IL-17A-CD4+ cells in peripheral blood lymphocytes in the 10 MS patients who had received IFNβ than in the 24 MS patients who had not received IFNβ or the 15 healthy controls (P < 0.05). The seven MS patients who received IFNβ showed significantly lower IL-17A levels in CSF than did the 27 MS patients who had not received IFNβ (P < 0.05).Conclusions The present results suggest the suppression of IL-17A production in the central nervous system and augmentation of Th9 cells in the peripheral blood by IFNβ in MS patients.",
author = "Hikaru Doi and Song, {Zi Ye} and Satoshi Yoshimura and Takahisa Tateishi and Tomomi Yonekawa and Ryo Yamasaki and Hiroyuki Murai and Takuya Matsushita and Jun-Ichi Kira",
year = "2014",
month = "1",
day = "1",
doi = "10.1111/cen3.12138",
language = "English",
volume = "5",
pages = "321--327",
journal = "Clinical and Experimental Neuroimmunology",
issn = "1759-1961",
publisher = "John Wiley and Sons Ltd",
number = "3",

}

TY - JOUR

T1 - Distinct cytokine and T helper cell profiles between patients with multiple sclerosis who had or had not received interferon-beta

AU - Doi, Hikaru

AU - Song, Zi Ye

AU - Yoshimura, Satoshi

AU - Tateishi, Takahisa

AU - Yonekawa, Tomomi

AU - Yamasaki, Ryo

AU - Murai, Hiroyuki

AU - Matsushita, Takuya

AU - Kira, Jun-Ichi

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Objectives Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system, and is generally considered to be mediated by T helper (Th) 1/Th17 cells. Interferon-β (IFNβ) is widely used as a disease-modifying MS drug, but its effects on Th17 cells are still disputed. Furthermore, the effects of IFNβ on Th9 cells have not been elucidated. The present study aimed to clarify the effects of IFNβ on cytokines/growth factors in cerebrospinal fluid (CSF) and cytokine-producing Th cells in peripheral blood.Methods First, the frequency of IFNγ, interleukin (IL)-17A, IL-9, and IL-4-producing Th cells in peripheral blood lymphocytes was analyzed by flow cytometry in 34 MS patients and 15 healthy volunteers enrolled in the cytokine-producing Th cell study. Second, levels of 27 cytokines/growth factors in the CSF were measured using a multiple fluorescence bead-based immunoassay in 34 MS patients enrolled in the cytokines/growth factors study.Results We found a significantly higher frequency of IL-4-IL-9+CD4+ T cells and lower frequency of IFNγ+IL-17A-CD4+ cells in peripheral blood lymphocytes in the 10 MS patients who had received IFNβ than in the 24 MS patients who had not received IFNβ or the 15 healthy controls (P < 0.05). The seven MS patients who received IFNβ showed significantly lower IL-17A levels in CSF than did the 27 MS patients who had not received IFNβ (P < 0.05).Conclusions The present results suggest the suppression of IL-17A production in the central nervous system and augmentation of Th9 cells in the peripheral blood by IFNβ in MS patients.

AB - Objectives Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system, and is generally considered to be mediated by T helper (Th) 1/Th17 cells. Interferon-β (IFNβ) is widely used as a disease-modifying MS drug, but its effects on Th17 cells are still disputed. Furthermore, the effects of IFNβ on Th9 cells have not been elucidated. The present study aimed to clarify the effects of IFNβ on cytokines/growth factors in cerebrospinal fluid (CSF) and cytokine-producing Th cells in peripheral blood.Methods First, the frequency of IFNγ, interleukin (IL)-17A, IL-9, and IL-4-producing Th cells in peripheral blood lymphocytes was analyzed by flow cytometry in 34 MS patients and 15 healthy volunteers enrolled in the cytokine-producing Th cell study. Second, levels of 27 cytokines/growth factors in the CSF were measured using a multiple fluorescence bead-based immunoassay in 34 MS patients enrolled in the cytokines/growth factors study.Results We found a significantly higher frequency of IL-4-IL-9+CD4+ T cells and lower frequency of IFNγ+IL-17A-CD4+ cells in peripheral blood lymphocytes in the 10 MS patients who had received IFNβ than in the 24 MS patients who had not received IFNβ or the 15 healthy controls (P < 0.05). The seven MS patients who received IFNβ showed significantly lower IL-17A levels in CSF than did the 27 MS patients who had not received IFNβ (P < 0.05).Conclusions The present results suggest the suppression of IL-17A production in the central nervous system and augmentation of Th9 cells in the peripheral blood by IFNβ in MS patients.

UR - http://www.scopus.com/inward/record.url?scp=84908153071&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84908153071&partnerID=8YFLogxK

U2 - 10.1111/cen3.12138

DO - 10.1111/cen3.12138

M3 - Article

AN - SCOPUS:84908153071

VL - 5

SP - 321

EP - 327

JO - Clinical and Experimental Neuroimmunology

JF - Clinical and Experimental Neuroimmunology

SN - 1759-1961

IS - 3

ER -