Distinct roles of protein disulfide isomerase and P5 sulfhydryl oxidoreductases in multiple pathways for oxidation of structurally diverse storage proteins in rice

Yayoi Onda, Ai Nagamine, Mutsumi Sakurai, Toshihiro Kumamaru, Masahiro Ogawa, Yasushi Kawagoe

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

In the rice (Oryza sativa) endosperm, storage proteins are synthesized on the rough endoplasmic reticulum (ER), in which prolamins are sorted to protein bodies (PBs) called type-I PB (PB-I). Protein disulfide isomerase (PDI) family oxidoreductase PDIL2;3, an ortholog of human P5, contains a conserved structural disulfide in the redox-inactive thioredoxin-like (TRX) domain and was efficiently targeted to the surface of PB-I in a redox active site-dependent manner, whereas PDIL1;1, an ortholog of human PDI, was localized in the ER lumen. Complementation analyses using PDIL1;1 knockout esp2 mutant indicated that the a and a′ TRX domains of PDIL1;1 exhibited similar redox activities and that PDIL2;3 was unable to perform the PDIL1;1 functions. PDIL2;3 knockdown inhibited the accumulation of Cys-rich 10-kD prolamin (crP10) in the core of PB-I. Conversely, crP10 knockdown dispersed PDIL2;3 into the ER lumen. Glutathione S-transferase-PDIL2;3 formed a stable tetramer when it was expressed in Escherichia coli, and the recombinant PDIL2;3 tetramer facilitated a-globulin(C79F) mutant protein to form nonnative intermolecular disulfide bonds in vitro. These results indicate that PDIL2;3 and PDIL1;1 are not functionally redundant in sulfhydryl oxidations of structurally diverse storage proteins and play distinct roles in PB development. We discuss PDIL2;3-dependent and PDIL2;3-independent oxidation pathways that sustain disulfide bonds of crP10 in PB-I.

Original languageEnglish
Pages (from-to)210-223
Number of pages14
JournalPlant Cell
Volume23
Issue number1
DOIs
Publication statusPublished - Jan 1 2011

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protein disulfide-isomerase
Protein Disulfide-Isomerases
protein bodies
oxidoreductases
storage proteins
Oxidoreductases
oxidation
rice
Proteins
Prolamins
Disulfides
prolamins
Oxidation-Reduction
disulfide bonds
Thioredoxins
endoplasmic reticulum
Endoplasmic Reticulum
knockout mutants
rough endoplasmic reticulum
body protein

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

Distinct roles of protein disulfide isomerase and P5 sulfhydryl oxidoreductases in multiple pathways for oxidation of structurally diverse storage proteins in rice. / Onda, Yayoi; Nagamine, Ai; Sakurai, Mutsumi; Kumamaru, Toshihiro; Ogawa, Masahiro; Kawagoe, Yasushi.

In: Plant Cell, Vol. 23, No. 1, 01.01.2011, p. 210-223.

Research output: Contribution to journalArticle

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