Distinctive expression of extracellular matrix molecules at mRNA and protein levels during formation of cellular and acellular cementum in the rat

Yasuyuki Sasano, Yuriko Maruya, Hidenori Sato, Jing Xu Zhu, Ichiro Takahashi, Itaru Mizoguchi, Manabu Kagayama

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Little is known about differential expression of extracellular matrices secreted by cementoblasts between cellular and acellular cementum. We hypothesize that cementoblasts lining acellular cementum express extracellular matrix genes differently from those lining cellular cementum, thereby forming two distinct types of extracellular matrices. To test this hypothesis, we investigated spatial and temporal gene expression of selected extracellular matrix molecules, that is type I collagen, bone sialoprotein, osteocalcin and osteopontin, during formation of both cellular and acellular cementum using in situ hybridization. In addition, their extracellularly deposited and accumulated proteins were examined immunohistochemically. The mRNA transcripts of pro-α1(I) collagen were primarily localized in cementoblasts of cellular cementum and cementocytes, while those of bone sialoprotein were predominantly seen in cementoblasts lining acellular cementum. In contrast, osteocalcin was expressed by both types of cementoblasts and cementocytes and so was osteopontin but only transiently. Our immunohistochemical examination revealed that translated proteins were localized extracellularly where the genes had been expressed intracellularly. The present study demonstrated the distinctive expression of genes and proteins of the extracellular matrix molecules between cellular and acellular cementum.

Original languageEnglish
Pages (from-to)91-99
Number of pages9
JournalHistochemical Journal
Volume33
Issue number2
DOIs
Publication statusPublished - Jan 1 2001

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Dental Cementum
Extracellular Matrix
Messenger RNA
Proteins
Osteopontin
Osteocalcin
Integrin-Binding Sialoprotein
Collagen Type I
Genes
In Situ Hybridization
Collagen

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Cell Biology

Cite this

Distinctive expression of extracellular matrix molecules at mRNA and protein levels during formation of cellular and acellular cementum in the rat. / Sasano, Yasuyuki; Maruya, Yuriko; Sato, Hidenori; Zhu, Jing Xu; Takahashi, Ichiro; Mizoguchi, Itaru; Kagayama, Manabu.

In: Histochemical Journal, Vol. 33, No. 2, 01.01.2001, p. 91-99.

Research output: Contribution to journalArticle

Sasano, Yasuyuki ; Maruya, Yuriko ; Sato, Hidenori ; Zhu, Jing Xu ; Takahashi, Ichiro ; Mizoguchi, Itaru ; Kagayama, Manabu. / Distinctive expression of extracellular matrix molecules at mRNA and protein levels during formation of cellular and acellular cementum in the rat. In: Histochemical Journal. 2001 ; Vol. 33, No. 2. pp. 91-99.
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