Distinguishable live erythroid and myeloid cells in β-globin ECFP x lysozyme EGFP mice

Susanne Heck, Olga Ermakova, Hiromi Iwasaki, Koichi Akashi, Chiao Wang Sun, Thomas M. Ryan, Tim Townes, Thomas Graf

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We previously described a mouse line that contains green myelomonocytic cells due to the knock-in of enhanced green fluorescence protein (EGFP) into the lysozyme M gene. We have now created a transgenic line with fluorescent erythroid cells using a β-globin locus control region driving the enhanced cyan fluorescence protein (ECFP) gene. These mice exhibit cyan fluorescent cells specifically in the erythroid compartment and in megakaryocyte-erythroid progenitors. Crossing the animals with lysozyme EGFP mice yielded a line in which live erythroid and myeloid cells can readily be distinguished by fluorescence microscopy and by fluorescence-activated cell-sorter scanner. This cross allowed unambiguous identification of unstained mixed erythroid-myeloid colonies for the first time. The new mouse lines should become useful tools to dissect the branching between erythroid and myelomonocytic cells during in vitro differentiation of definitive multipotent progenitors.

Original languageEnglish
Pages (from-to)903-906
Number of pages4
JournalBlood
Volume101
Issue number3
DOIs
Publication statusPublished - Feb 1 2003

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Erythroid Cells
Globins
Myeloid Cells
Muramidase
Fluorescence
Megakaryocyte-Erythroid Progenitor Cells
Proteins
Locus Control Region
Fluorescence microscopy
Fluorescence Microscopy
Animals
Genes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Heck, S., Ermakova, O., Iwasaki, H., Akashi, K., Sun, C. W., Ryan, T. M., ... Graf, T. (2003). Distinguishable live erythroid and myeloid cells in β-globin ECFP x lysozyme EGFP mice. Blood, 101(3), 903-906. https://doi.org/10.1182/blood-2002-06-1861

Distinguishable live erythroid and myeloid cells in β-globin ECFP x lysozyme EGFP mice. / Heck, Susanne; Ermakova, Olga; Iwasaki, Hiromi; Akashi, Koichi; Sun, Chiao Wang; Ryan, Thomas M.; Townes, Tim; Graf, Thomas.

In: Blood, Vol. 101, No. 3, 01.02.2003, p. 903-906.

Research output: Contribution to journalArticle

Heck, S, Ermakova, O, Iwasaki, H, Akashi, K, Sun, CW, Ryan, TM, Townes, T & Graf, T 2003, 'Distinguishable live erythroid and myeloid cells in β-globin ECFP x lysozyme EGFP mice', Blood, vol. 101, no. 3, pp. 903-906. https://doi.org/10.1182/blood-2002-06-1861
Heck, Susanne ; Ermakova, Olga ; Iwasaki, Hiromi ; Akashi, Koichi ; Sun, Chiao Wang ; Ryan, Thomas M. ; Townes, Tim ; Graf, Thomas. / Distinguishable live erythroid and myeloid cells in β-globin ECFP x lysozyme EGFP mice. In: Blood. 2003 ; Vol. 101, No. 3. pp. 903-906.
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