Distribution and localization of glia fibrillary acidic protein in colons affected by Hirschsprung's disease

T. Kawana, O. Nada, K. Ikeda, S. Goto, R. Hirose, T. Taguchi, M. Kubota, T. Toyohara, T. Shono

Research output: Contribution to journalArticle

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Abstract

The distribution and localization of glial fibrillary acidic (GFA) protein were examined by means of immunohistochemistry in normoganglionic, oligoganglinonic, and aganglionic segments of colons from 25 patients with Hirschsprung's disease, including four cases of long segment aganglionosis. In normoganglionic segments, GFA proteinpositive glial cells were densely distributed within the myenteric plexus, but sparse in the submucous plexus. Aganglionic segments were completely devoid of glial cells with GFA protein immunoreactivity, coinciding with the lack of enteric ganglia. Instead, GFA protein was found specifically in association with the hypertrophic nerve fasciculi and their branches, which were mainly located in the intermuscular zone and submucosal connective tissue in the distal aganglionic segment of diseased bowels. However, two types of short and long segment aganglionosis differed in the distribution pattern of GFA protein; the extrinsic nerve fasciculi in short segment disease extended toward the normoganglionic segment, but in long segment disease they did not reach this area. A moderate number of GFA protein-positive fasciculi were observed within the circular muscle layer of proximal aganglionosis, while no immunoreactive fasciculi were encountered within the circular muscle layer of the corresponding parts in long segment aganglionosis. Immunohistochemistry for GFA protein can be of excellent diagnostic value for the aganglionic colon with Hirschsprung's disease, since GFA protein immunohistochemistry discloses exclusively extrinsic, hypertrophic nerve fasciculi, characteristic of the bowel in cases of Hirschsprung's disease.

Original languageEnglish
Pages (from-to)448-452
Number of pages5
JournalJournal of Pediatric Surgery
Volume24
Issue number5
DOIs
Publication statusPublished - May 1989
Externally publishedYes

Fingerprint

Hirschsprung Disease
Glial Fibrillary Acidic Protein
Neuroglia
Colon
Proteins
Immunohistochemistry
Submucous Plexus
Myenteric Plexus
Muscles
Ganglia
Connective Tissue

All Science Journal Classification (ASJC) codes

  • Surgery
  • Pediatrics, Perinatology, and Child Health

Cite this

Distribution and localization of glia fibrillary acidic protein in colons affected by Hirschsprung's disease. / Kawana, T.; Nada, O.; Ikeda, K.; Goto, S.; Hirose, R.; Taguchi, T.; Kubota, M.; Toyohara, T.; Shono, T.

In: Journal of Pediatric Surgery, Vol. 24, No. 5, 05.1989, p. 448-452.

Research output: Contribution to journalArticle

Kawana, T. ; Nada, O. ; Ikeda, K. ; Goto, S. ; Hirose, R. ; Taguchi, T. ; Kubota, M. ; Toyohara, T. ; Shono, T. / Distribution and localization of glia fibrillary acidic protein in colons affected by Hirschsprung's disease. In: Journal of Pediatric Surgery. 1989 ; Vol. 24, No. 5. pp. 448-452.
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