Distribution of p27(KIP1), cylin D1, and proliferating cell nuclear antigen after retinal detachment

Kazuhiko Yoshida, Satoru Kase, Keiko Nakayama, Hiroyasu Nagahama, Takayuki Harada, Hiromi Ikeda, Chikako Harada, Junko Imaki, Kazuhiro Ohgami, Kenji Shiratori, Shigeaki Ohno, Keiichi Nakayama

Research output: Contribution to journalArticle

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Abstract

Purpose: To examine the expression of the p27(KIP1), cyclin D1, and proliferating cell nuclear antigen (PCNA) in the retina and retinal pigment epithelium (RPE) after retinal detachment. Methods: Normal eyes and eyes at 2 or 4 days after retinal detachment with the C57B16 mouse were analyzed by immunocytochemistry using anti-p27(KIP1), anti-cyclin D1, and anti-proliferating cell nuclear antigen (PCNA) antibodies as well as anti-glutamate synthetase (GS) antibody. Results: fhe p27(KIP1) positive nuclei were distributed in the inner nuclear layer (INL) and the RPE of the normal mice eye. In the INL, p27(KIP1) was detected in the middle sublayer, where the nuclei of glutamate synthetase positive Müller cells were situated. In contrast, cyclin D1 was not detected either in the retina or in the RPE. At 2 and 4 days after the retinal detachment, RPE cells under the detached retina were negative for p27(KIP1) and positive for cyclin D1 and PCNA. In the INL of the detached retina, p27(KIP1) was detected after 2 days, but was not detected after 4 days. In contrast, PCNA was not detected in the INL after 2 days, but was detected after 4 days. Cyclin D1 was detected in the middle sublayer of the INL at both 2 and 4 days after the retinal detachment. Conclusion: These results suggested that degradation of p27(KIP1) and expression of cyclin D1 was involved in the proliferation of the Müller cells as well as RPE cells after retinal detachment.

Original languageEnglish
Pages (from-to)437-441
Number of pages5
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Volume242
Issue number5
DOIs
Publication statusPublished - May 1 2004

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Cyclin D1
Proliferating Cell Nuclear Antigen
Retinal Detachment
Retinal Pigment Epithelium
Retina
Ligases
Glutamic Acid
Antibodies
Immunohistochemistry
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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Distribution of p27(KIP1), cylin D1, and proliferating cell nuclear antigen after retinal detachment. / Yoshida, Kazuhiko; Kase, Satoru; Nakayama, Keiko; Nagahama, Hiroyasu; Harada, Takayuki; Ikeda, Hiromi; Harada, Chikako; Imaki, Junko; Ohgami, Kazuhiro; Shiratori, Kenji; Ohno, Shigeaki; Nakayama, Keiichi.

In: Graefe's Archive for Clinical and Experimental Ophthalmology, Vol. 242, No. 5, 01.05.2004, p. 437-441.

Research output: Contribution to journalArticle

Yoshida, K, Kase, S, Nakayama, K, Nagahama, H, Harada, T, Ikeda, H, Harada, C, Imaki, J, Ohgami, K, Shiratori, K, Ohno, S & Nakayama, K 2004, 'Distribution of p27(KIP1), cylin D1, and proliferating cell nuclear antigen after retinal detachment', Graefe's Archive for Clinical and Experimental Ophthalmology, vol. 242, no. 5, pp. 437-441. https://doi.org/10.1007/s00417-004-0861-7
Yoshida, Kazuhiko ; Kase, Satoru ; Nakayama, Keiko ; Nagahama, Hiroyasu ; Harada, Takayuki ; Ikeda, Hiromi ; Harada, Chikako ; Imaki, Junko ; Ohgami, Kazuhiro ; Shiratori, Kenji ; Ohno, Shigeaki ; Nakayama, Keiichi. / Distribution of p27(KIP1), cylin D1, and proliferating cell nuclear antigen after retinal detachment. In: Graefe's Archive for Clinical and Experimental Ophthalmology. 2004 ; Vol. 242, No. 5. pp. 437-441.
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abstract = "Purpose: To examine the expression of the p27(KIP1), cyclin D1, and proliferating cell nuclear antigen (PCNA) in the retina and retinal pigment epithelium (RPE) after retinal detachment. Methods: Normal eyes and eyes at 2 or 4 days after retinal detachment with the C57B16 mouse were analyzed by immunocytochemistry using anti-p27(KIP1), anti-cyclin D1, and anti-proliferating cell nuclear antigen (PCNA) antibodies as well as anti-glutamate synthetase (GS) antibody. Results: fhe p27(KIP1) positive nuclei were distributed in the inner nuclear layer (INL) and the RPE of the normal mice eye. In the INL, p27(KIP1) was detected in the middle sublayer, where the nuclei of glutamate synthetase positive M{\"u}ller cells were situated. In contrast, cyclin D1 was not detected either in the retina or in the RPE. At 2 and 4 days after the retinal detachment, RPE cells under the detached retina were negative for p27(KIP1) and positive for cyclin D1 and PCNA. In the INL of the detached retina, p27(KIP1) was detected after 2 days, but was not detected after 4 days. In contrast, PCNA was not detected in the INL after 2 days, but was detected after 4 days. Cyclin D1 was detected in the middle sublayer of the INL at both 2 and 4 days after the retinal detachment. Conclusion: These results suggested that degradation of p27(KIP1) and expression of cyclin D1 was involved in the proliferation of the M{\"u}ller cells as well as RPE cells after retinal detachment.",
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T1 - Distribution of p27(KIP1), cylin D1, and proliferating cell nuclear antigen after retinal detachment

AU - Yoshida, Kazuhiko

AU - Kase, Satoru

AU - Nakayama, Keiko

AU - Nagahama, Hiroyasu

AU - Harada, Takayuki

AU - Ikeda, Hiromi

AU - Harada, Chikako

AU - Imaki, Junko

AU - Ohgami, Kazuhiro

AU - Shiratori, Kenji

AU - Ohno, Shigeaki

AU - Nakayama, Keiichi

PY - 2004/5/1

Y1 - 2004/5/1

N2 - Purpose: To examine the expression of the p27(KIP1), cyclin D1, and proliferating cell nuclear antigen (PCNA) in the retina and retinal pigment epithelium (RPE) after retinal detachment. Methods: Normal eyes and eyes at 2 or 4 days after retinal detachment with the C57B16 mouse were analyzed by immunocytochemistry using anti-p27(KIP1), anti-cyclin D1, and anti-proliferating cell nuclear antigen (PCNA) antibodies as well as anti-glutamate synthetase (GS) antibody. Results: fhe p27(KIP1) positive nuclei were distributed in the inner nuclear layer (INL) and the RPE of the normal mice eye. In the INL, p27(KIP1) was detected in the middle sublayer, where the nuclei of glutamate synthetase positive Müller cells were situated. In contrast, cyclin D1 was not detected either in the retina or in the RPE. At 2 and 4 days after the retinal detachment, RPE cells under the detached retina were negative for p27(KIP1) and positive for cyclin D1 and PCNA. In the INL of the detached retina, p27(KIP1) was detected after 2 days, but was not detected after 4 days. In contrast, PCNA was not detected in the INL after 2 days, but was detected after 4 days. Cyclin D1 was detected in the middle sublayer of the INL at both 2 and 4 days after the retinal detachment. Conclusion: These results suggested that degradation of p27(KIP1) and expression of cyclin D1 was involved in the proliferation of the Müller cells as well as RPE cells after retinal detachment.

AB - Purpose: To examine the expression of the p27(KIP1), cyclin D1, and proliferating cell nuclear antigen (PCNA) in the retina and retinal pigment epithelium (RPE) after retinal detachment. Methods: Normal eyes and eyes at 2 or 4 days after retinal detachment with the C57B16 mouse were analyzed by immunocytochemistry using anti-p27(KIP1), anti-cyclin D1, and anti-proliferating cell nuclear antigen (PCNA) antibodies as well as anti-glutamate synthetase (GS) antibody. Results: fhe p27(KIP1) positive nuclei were distributed in the inner nuclear layer (INL) and the RPE of the normal mice eye. In the INL, p27(KIP1) was detected in the middle sublayer, where the nuclei of glutamate synthetase positive Müller cells were situated. In contrast, cyclin D1 was not detected either in the retina or in the RPE. At 2 and 4 days after the retinal detachment, RPE cells under the detached retina were negative for p27(KIP1) and positive for cyclin D1 and PCNA. In the INL of the detached retina, p27(KIP1) was detected after 2 days, but was not detected after 4 days. In contrast, PCNA was not detected in the INL after 2 days, but was detected after 4 days. Cyclin D1 was detected in the middle sublayer of the INL at both 2 and 4 days after the retinal detachment. Conclusion: These results suggested that degradation of p27(KIP1) and expression of cyclin D1 was involved in the proliferation of the Müller cells as well as RPE cells after retinal detachment.

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