TY - JOUR
T1 - Distribution of proglucagon mRNA and GLP-1 in the brainstem of chicks
AU - Tachibana, Tetsuya
AU - Hiramatsu, Kohzy
AU - Furuse, Mitsuhiro
AU - Hasegawa, Shin
AU - Yoshizawa, Fumiaki
AU - Sugahara, Kunio
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Young Scientists (No. 15780187) and a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2005/2
Y1 - 2005/2
N2 - Glucagon-like peptide-1 (GLP-1), structurally similar to glucagon, synthesized from the precursor proglucagon, is a well known anorexigenic peptide in the brain of several animal species. However, there are no previous reports concerning GLP-1-containing neurons in the chick brain. The aim of the present study was to investigate the distribution of proglucagon mRNA and GLP-1-immunoreactive (GLI) perikarya in various regions of the chick brain. We detected proglucagon mRNA in the brainstem, and to a lesser extent in the telencephalon. In the brainstem, a study using immunohistochemistry revealed that GLI perikarya were present in the nucleus motorius nervi facialis pars dosalis, nucleus motoris dorsalis nervi vagi and nucleus tractus solitarii. Furthermore, we found that proglucagon mRNA expression in the brainstem decreased after 24 h fasting. The present findings support the idea that endogenous GLP-1 is involved in feeding behavior of chicks.
AB - Glucagon-like peptide-1 (GLP-1), structurally similar to glucagon, synthesized from the precursor proglucagon, is a well known anorexigenic peptide in the brain of several animal species. However, there are no previous reports concerning GLP-1-containing neurons in the chick brain. The aim of the present study was to investigate the distribution of proglucagon mRNA and GLP-1-immunoreactive (GLI) perikarya in various regions of the chick brain. We detected proglucagon mRNA in the brainstem, and to a lesser extent in the telencephalon. In the brainstem, a study using immunohistochemistry revealed that GLI perikarya were present in the nucleus motorius nervi facialis pars dosalis, nucleus motoris dorsalis nervi vagi and nucleus tractus solitarii. Furthermore, we found that proglucagon mRNA expression in the brainstem decreased after 24 h fasting. The present findings support the idea that endogenous GLP-1 is involved in feeding behavior of chicks.
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U2 - 10.1016/j.cbpb.2005.01.001
DO - 10.1016/j.cbpb.2005.01.001
M3 - Article
C2 - 15748860
AN - SCOPUS:14644412372
SN - 1095-6433
VL - 140
SP - 203
EP - 207
JO - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
IS - 2
ER -