Distribution of Type I and Type III collagen in the Developing Periodontal Ligament of Mice

Yu Hsin Huang, Yasuyoshi Ohsaki, Kojiro Kurisu

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Abstract

In order to localize type I and type III collagen in developing periodontal ligament, immunofluorescent and immunoelectron microscopic examinations were undertaken. The materials used were the maxillary molars of CH mice, 13 days to 6 months old. The antibodies used were monospecific polyclonal antibodies against type I or type III collagen raised in rabbits or guinea pigs, respectively. Single- and double-staining methods were employed in immunofluorescent as well as immunoelectron microscopic examinations. In immunofluorescent examinations, during the development of the periodontal ligament, periodontal fibers showed intense and homogenous staining for both type I and type III collagen; while Sharpey's fibers in the alveolar bone showed a heterogenous staining in cross and longitudinal sections for both types of collagen. In immunoelectron microscopic examinations, fibrils of periodontal fibers showed positive staining for type I and type III collagen simultaneously and had characteristic cross-banding and had a large diameter (ranging 40 ∼ 80 nm) which remained constant during development. Sharpey's fibers in the alveolar bone or in the cementum showed the same staining pattern as the periodontal fibers, except for an afibrillar area which showed negative staining for both type I and type III collagen. The results obtained suggest that periodontal fibers and Sharpey's fibers consist of cofibrils of at least type I and type III collagen.

Original languageEnglish
Pages (from-to)25-35
Number of pages11
JournalMatrix
Volume11
Issue number1
DOIs
Publication statusPublished - Jan 1 1991

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Periodontal Ligament
Collagen Type III
Collagen Type I
Staining and Labeling
Dental Cementum
Bone and Bones
Negative Staining
Antibodies
Guinea Pigs
Collagen
Rabbits

All Science Journal Classification (ASJC) codes

  • Rheumatology

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Distribution of Type I and Type III collagen in the Developing Periodontal Ligament of Mice. / Huang, Yu Hsin; Ohsaki, Yasuyoshi; Kurisu, Kojiro.

In: Matrix, Vol. 11, No. 1, 01.01.1991, p. 25-35.

Research output: Contribution to journalArticle

Huang, Yu Hsin ; Ohsaki, Yasuyoshi ; Kurisu, Kojiro. / Distribution of Type I and Type III collagen in the Developing Periodontal Ligament of Mice. In: Matrix. 1991 ; Vol. 11, No. 1. pp. 25-35.
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