Disturbance of regulation of sodium by cis-diamminedichloroplatinum in perilymph of the guinea pig cochlea

Shizuo Komune, Takashi Nakagawa, Akira Inokuchi, Takashi Kimitsuki, Sohtaro Komiyama, Kenji Matsuda, Kazutaka Hisashi, Toshimitsu Kobayashi

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Abstract

We studied the acute effects of cis-diamminedichloroplatinum (CDDP) on the cochlear partition and inner ear fluid in the guinea pig. At 48 hours after the administration of a single intramuscular injection of CDDP, 12.5 mg/kg of body weight, the endocochlear resting potential (EP) was significantly decreased to 32.1 ± 1.8 mV in the treated animals, versus 80.6 ±.1.0 mV in the control animals. There was a significant rise in potassium (K+), sodium (Na+), and chlorine (Cl) in the endolymph of the animals treated with CDDP as compared with the control animals. Only Na+ was found to increase significantly in the perilymph, reaching more than twice the level of the control animals; both K+ and Cl remained within the normal range. Serum electrolytes also remained within the normal range. Evaluation of modified ionic permeabilities across the endolymph-perilymph barrier showed an apparent increase in Na+ permeability and a normal range of K+ and Cl permeabilities. Histopathologic examination of the cochlea showed a moderate collapse of the endolymphatic space, with atrophy of the stria vascularis and destruction of the outer hair cells. The findings suggest that the acute changes produced in the cochlea by administration of CDDP were attributable to a breakdown in the regulation of Na+ metabolism in the perilymph.

Original languageEnglish
Pages (from-to)149-154
Number of pages6
JournalAnnals of Otology, Rhinology & Laryngology
Volume104
Issue number2
DOIs
Publication statusPublished - Feb 1995

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All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

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