Diversity in butane monooxygenases among butane-grown bacteria

Natsuko Hamamura, Ryan T. Storfa, Lewis Semprini, Daniel J. Arp

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Butane monooxygenases of butane-grown Pseudomonas butanovora, Mycobacterium vaccae JOB5, and an environmental isolate, CF8, were compared at the physiological level. The presence of butane monooxygenases in these bacteria was indicated by the following results. (i) O2 was required for butane degradation. (ii) 1-Butanol was produced during butane degradation. (iii) Acetylene inhibited both butane oxidation and 1-butanol production. The responses to the known monooxygenase inactivator, ethylene, and inhibitor, allyl thiourea (ATU), discriminated butane degradation among the three bacteria. Ethylene irreversibly inactivated butane oxidation by P. butanovora but not by M. vaccae or CF8. In contrast, butane oxidation by only CF8 was strongly inhibited by ATU. In all three strains of butane-grown bacteria, specific polypeptides were labeled in the presence of [14C]acetylene. The [14C]acetylene labeling patterns were different among the three bacteria. Exposure of lactate-grown CF8 and P. butanovora and glucose-grown M. vaccae to butane induced butane oxidation activity as well as the specific acetylene-binding polypeptides. Ammonia was oxidized by all three bacteria. P. butanovora oxidized ammonia to hydroxylamine, while CF8 and M. vaccae produced nitrite. All three bacteria oxidized ethylene to ethylene oxide. Methane oxidation was not detected by any of the bacteria. The results indicate the presence of three distinct butane monooxygenases in butane-grown P. butanovora, M. vaccae, and CF8.

Original languageEnglish
Pages (from-to)4586-4593
Number of pages8
JournalApplied and environmental microbiology
Volume65
Issue number10
Publication statusPublished - Jan 1 1999
Externally publishedYes

Fingerprint

Mixed Function Oxygenases
Pseudomonas butanovora
Bacteria
Mycobacterium vaccae
acetylene
bacterium
bacteria
ethylene
oxidation
Acetylene
degradation
ammonia
thiourea
Thiourea
1-Butanol
butanol
butanes
butane
Ammonia
nitrite

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Hamamura, N., Storfa, R. T., Semprini, L., & Arp, D. J. (1999). Diversity in butane monooxygenases among butane-grown bacteria. Applied and environmental microbiology, 65(10), 4586-4593.

Diversity in butane monooxygenases among butane-grown bacteria. / Hamamura, Natsuko; Storfa, Ryan T.; Semprini, Lewis; Arp, Daniel J.

In: Applied and environmental microbiology, Vol. 65, No. 10, 01.01.1999, p. 4586-4593.

Research output: Contribution to journalArticle

Hamamura, N, Storfa, RT, Semprini, L & Arp, DJ 1999, 'Diversity in butane monooxygenases among butane-grown bacteria', Applied and environmental microbiology, vol. 65, no. 10, pp. 4586-4593.
Hamamura, Natsuko ; Storfa, Ryan T. ; Semprini, Lewis ; Arp, Daniel J. / Diversity in butane monooxygenases among butane-grown bacteria. In: Applied and environmental microbiology. 1999 ; Vol. 65, No. 10. pp. 4586-4593.
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