Diversity in virulence of Xanthomonas oryzae pv. Oryzae from Northern Vietnam

Naruto Furuya, Satoru Taura, Takahiro Goto, Bui Trong Thuy, Phan Huu Ton, Kenichi Tsuchiya, Atsushi Yoshimura

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A survey of the geographical distribution of pathogenic races of Xanthomonas oryzae pv. oryzae (Xoo) causing bacterial blight (BB) of rice in northern Vietnam was conducted. We characterized strains of Xoo collected from nine regions in this part of Vietnam in 2001 and 2002 using polymerase chain reaction (PCR)-based DNA fingerprinting and virulence analysis. The pathogenicity of Vietnamese 84 strains was tested for their virulence to BB near-isogenic lines with a single resistance gene. Based on virulence analysis using 11 differential lines (IR24, IRBB1, IRBB2, IRBB3, IRBB4, IRBB5, IRBB 7, IRBB10, IRBB11, IRBB21 and Taichung Native 1), each harboring a single resistance gene, four pathotypes (tentatively designated as races G1 to G4) were identified. All strains were virulent to the resistance genes Xa1, Xa2, Xa10, Xa11, Xa14 and Xa18, and xa5 and Xa21 were effective against a major portion of the Vietnamese Xoo population; followed by Xa7. Most of the Vietnamese hybrid and improved varieties cultivated in northern Vietnam were susceptible to all races and no Vietnamese variety was resistant to all races. Our study revealed that strains from northern Vietnam comprised two major races (G2 and G3) and two minor races (G1 and G4), which were widely distributed and detected at lower frequencies, respectively, in northern Vietnam. Seventy six strains of Xoo were analyzed by DNA fingerprinting using two PCR-based assays. Two groups, designated as clusters I and II, were respectively identified, in which cluster I contained race G4 strains and cluster II race G1 strains. Combining DNA and pathotype analysis, we hereby present the situation of Xoo recently isolated in northern Vietnam.

Original languageEnglish
Pages (from-to)329-338
Number of pages10
JournalJapan Agricultural Research Quarterly
Volume46
Issue number4
DOIs
Publication statusPublished - Dec 1 2012

Fingerprint

Xanthomonas
Xanthomonas oryzae pv. oryzae
Vietnam
virulence
Virulence
pathotype
DNA fingerprinting
DNA Fingerprinting
pathotypes
blight
polymerase chain reaction
gene
Genes
Polymerase Chain Reaction
genes
Oryza
pathogenicity
isogenic lines
geographical distribution
rice

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Ecology
  • Animal Science and Zoology
  • Agronomy and Crop Science

Cite this

Furuya, N., Taura, S., Goto, T., Thuy, B. T., Ton, P. H., Tsuchiya, K., & Yoshimura, A. (2012). Diversity in virulence of Xanthomonas oryzae pv. Oryzae from Northern Vietnam. Japan Agricultural Research Quarterly, 46(4), 329-338. https://doi.org/10.6090/jarq.46.329

Diversity in virulence of Xanthomonas oryzae pv. Oryzae from Northern Vietnam. / Furuya, Naruto; Taura, Satoru; Goto, Takahiro; Thuy, Bui Trong; Ton, Phan Huu; Tsuchiya, Kenichi; Yoshimura, Atsushi.

In: Japan Agricultural Research Quarterly, Vol. 46, No. 4, 01.12.2012, p. 329-338.

Research output: Contribution to journalArticle

Furuya, N, Taura, S, Goto, T, Thuy, BT, Ton, PH, Tsuchiya, K & Yoshimura, A 2012, 'Diversity in virulence of Xanthomonas oryzae pv. Oryzae from Northern Vietnam', Japan Agricultural Research Quarterly, vol. 46, no. 4, pp. 329-338. https://doi.org/10.6090/jarq.46.329
Furuya, Naruto ; Taura, Satoru ; Goto, Takahiro ; Thuy, Bui Trong ; Ton, Phan Huu ; Tsuchiya, Kenichi ; Yoshimura, Atsushi. / Diversity in virulence of Xanthomonas oryzae pv. Oryzae from Northern Vietnam. In: Japan Agricultural Research Quarterly. 2012 ; Vol. 46, No. 4. pp. 329-338.
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