TY - JOUR
T1 - Diversity of complement factor B/C2 in the common carp (Cyprinus carpio)
T2 - Three isotypes of B/C2-A expressed in different tissues
AU - Nakao, Miki
AU - Matsumoto, Momoe
AU - Nakazawa, Mika
AU - Fujiki, Kazuhiro
AU - Yano, Tomoki
N1 - Funding Information:
We are grateful to Dr C.J. Bayne, Oregon State University, for critically reading the manuscript. This study was supported partly by a grant-in-aid for scientific research from the Japanese Ministry of Education, Science, Sports and Culture (12660173 to M.N., 11694220 and 11460098 to T.Y.).
PY - 2002
Y1 - 2002
N2 - Complement factor B and C2 are two critical proteases for complement activation. Some bony fish have been reported to possess duplicated genes for B/C2, but there is no direct evidence regarding possible functional divergence. Here, we report the isolation of the second and third isotypes of carp B/C2-A, a close relative of other bony fish B reported to date, and designated B/C2-A2 and B/C2-A3. B/C2-A1 (previously reported B/C2-A) and B/C2-A2 share 78% amino acid identity and are synthesized mainly in hepatopancreas. On the other hand, B/C2-A3 showed less (∼60%) sequence identity with the other two isotypes. It was expressed mainly in kidney and spleen, and was up-regulated after injection of carp with scleroglucan or sodium alginate, known immunostimulants for fish. Phylogenetic analysis suggests that B/C2-A3 diverged before separation of carp and zebrafish. B/C2-A3 represent a novel B/C2-lineage functioning as an acute phase reactant in cyprinid fish.
AB - Complement factor B and C2 are two critical proteases for complement activation. Some bony fish have been reported to possess duplicated genes for B/C2, but there is no direct evidence regarding possible functional divergence. Here, we report the isolation of the second and third isotypes of carp B/C2-A, a close relative of other bony fish B reported to date, and designated B/C2-A2 and B/C2-A3. B/C2-A1 (previously reported B/C2-A) and B/C2-A2 share 78% amino acid identity and are synthesized mainly in hepatopancreas. On the other hand, B/C2-A3 showed less (∼60%) sequence identity with the other two isotypes. It was expressed mainly in kidney and spleen, and was up-regulated after injection of carp with scleroglucan or sodium alginate, known immunostimulants for fish. Phylogenetic analysis suggests that B/C2-A3 diverged before separation of carp and zebrafish. B/C2-A3 represent a novel B/C2-lineage functioning as an acute phase reactant in cyprinid fish.
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U2 - 10.1016/S0145-305X(01)00083-0
DO - 10.1016/S0145-305X(01)00083-0
M3 - Article
C2 - 12031413
AN - SCOPUS:0036258779
SN - 0145-305X
VL - 26
SP - 533
EP - 541
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
IS - 6
ER -