DNA analysis of the interspecific hybrid clones and the horticultural varieties of Chamaecyparis obtusa and C. pisifera

K. Narazaki, Atsushi Watanabe, K. Tomita, Y. Sasaki, S. Shiraishi

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Abstract

RAPD (random amplified polymorphic DNA) and SSCP (single strand conformation polymorphism) analyses were carried out to investigate the nuclear genome composition and chloroplast genome type of a natural hybrid and five horticultural varieties. In RAPD analysis, Fuji-2, a plus tree clone that is a natural hybrid between C. obtusa (SIEB. et ZUCC.) Endlicher and C. pisifera (SIEB. et ZUCC.) Endlicher, possessed all species-specific bands that were detected in these two species. It was reconfirmed at the DNA molecular level that this clone was an interspecific hybrid between them. SSCP analysis revealed that the chloroplast genome of Fuji-2 was C. obtusa type. This suggested that Fuji-2 was bred by crossing between C. pisifera as the seed parent and C. obtusa as the pollen parent. As no hybrids have ever been bred in this combination (C. pisifera × C. obtusa), a new possibility was shown in cross breeding of Chamaecyparis. In RAPD and SSCP analyses of horticultural varieties, each variety possessed species-specific bands of either C. obtusa or C. pisifera. The fact supported that they were mutants of C. obtusa or C. pisifera. From these results, DNA molecular markers such as RAPD and SSCP seem useful to identify the parental species of interspecific hybrid, and also useful to ascertain the origin of mutant.

Original languageEnglish
Pages (from-to)157-161
Number of pages5
JournalNihon Ringakkai Shi/Journal of the Japanese Forestry Society
Volume78
Issue number2
Publication statusPublished - Dec 1 1996

Fingerprint

Chamaecyparis pisifera
Chamaecyparis obtusa
clone
random amplified polymorphic DNA technique
single-stranded conformational polymorphism
clones
DNA
polymorphism
genome
chloroplast
Chamaecyparis
breeds
plus trees
mutants
nuclear genome
analysis
pollen
breeding
seed
genetic markers

All Science Journal Classification (ASJC) codes

  • Forestry

Cite this

DNA analysis of the interspecific hybrid clones and the horticultural varieties of Chamaecyparis obtusa and C. pisifera. / Narazaki, K.; Watanabe, Atsushi; Tomita, K.; Sasaki, Y.; Shiraishi, S.

In: Nihon Ringakkai Shi/Journal of the Japanese Forestry Society, Vol. 78, No. 2, 01.12.1996, p. 157-161.

Research output: Contribution to journalArticle

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abstract = "RAPD (random amplified polymorphic DNA) and SSCP (single strand conformation polymorphism) analyses were carried out to investigate the nuclear genome composition and chloroplast genome type of a natural hybrid and five horticultural varieties. In RAPD analysis, Fuji-2, a plus tree clone that is a natural hybrid between C. obtusa (SIEB. et ZUCC.) Endlicher and C. pisifera (SIEB. et ZUCC.) Endlicher, possessed all species-specific bands that were detected in these two species. It was reconfirmed at the DNA molecular level that this clone was an interspecific hybrid between them. SSCP analysis revealed that the chloroplast genome of Fuji-2 was C. obtusa type. This suggested that Fuji-2 was bred by crossing between C. pisifera as the seed parent and C. obtusa as the pollen parent. As no hybrids have ever been bred in this combination (C. pisifera × C. obtusa), a new possibility was shown in cross breeding of Chamaecyparis. In RAPD and SSCP analyses of horticultural varieties, each variety possessed species-specific bands of either C. obtusa or C. pisifera. The fact supported that they were mutants of C. obtusa or C. pisifera. From these results, DNA molecular markers such as RAPD and SSCP seem useful to identify the parental species of interspecific hybrid, and also useful to ascertain the origin of mutant.",
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