DNA binding affinity of pentapeptides selected from combinatorial library.

M. R. Alam, M. Maeda, S. Sasaki

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The combinatorial method has been applied to determine peptide ligands to the duplex DNA by using the solid-state pentapeptide library and the target-DNA conjugated magnetic beads. Seventy-one sequences were determined as ligands for AT duplex. Interestingly, hydrophobic amino acids such as Phe, Ile and Gly were most frequently determined. Relative binding affinity of the selected pentapeptides with the various DNA sequences was estimated by ethidium displacement assay in 10 mM SHE buffer. FQGII constituted of amino acids that were most frequently determined in the random screening showed highest binding affinity to the duplex DNA.

Original languageEnglish
Pages (from-to)173-174
Number of pages2
JournalNucleic acids symposium series
Issue number42
DOIs
Publication statusPublished - 1999

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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