DNA-binding domain of RCC1 protein is not essential for coupling mitosis with DNA replication

Hiroaki Seino, Naoki Hisamoto, Satoru Uzawa, Takeshi Sekiguchi, Takeharu Nishimoto

Research output: Contribution to journalArticle

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Abstract

The RCC1 protein that is required for coupling mitosis with the S phase has a DNA-binding domain in the N-terminal region outside the repeat. We found that RCC1 protein without any DNA-binding activity complemented the tsBN2 mutation with the same efficiency as that of intact RCC1 protein. In ts+ transformants of tsBN2 cells transfected with the RCC1 cDNA lacking the DNA-binding domain, an endogenous RCC1 disappeared at 39.5°C, and the deleted RCC1 protein encoded by the transfected cDNA was found in the cytoplasm, but a significant amount of it was also found in the nuclei. This deleted RCC1 protein was eluted from the nuclei with the same concentration of NaCl and DNase I as was used for the intact RCC1 protein in BHK21 cells. Furthermore, the deleted RCC1 protein co-migrated with the nucleosome fraction on sucrose density gradient analysis. These results indicate that the RCC1 protein binds chromatin with the aid of other unknown protein(s). Thus, the DNA-binding domain of RCC1 protein is not essential for coupling between the S and M phases, but was shown instead to function as a nuclear translocation signal.

Original languageEnglish
Pages (from-to)393-400
Number of pages8
JournalJournal of Cell Science
Volume102
Issue number3
Publication statusPublished - Jul 1992

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DNA Replication
Mitosis
DNA
Proteins
S Phase
Complementary DNA
Protein Domains
Nucleosomes
Deoxyribonuclease I
Cell Division
Chromatin
Sucrose
Cytoplasm
Mutation

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

DNA-binding domain of RCC1 protein is not essential for coupling mitosis with DNA replication. / Seino, Hiroaki; Hisamoto, Naoki; Uzawa, Satoru; Sekiguchi, Takeshi; Nishimoto, Takeharu.

In: Journal of Cell Science, Vol. 102, No. 3, 07.1992, p. 393-400.

Research output: Contribution to journalArticle

Seino, H, Hisamoto, N, Uzawa, S, Sekiguchi, T & Nishimoto, T 1992, 'DNA-binding domain of RCC1 protein is not essential for coupling mitosis with DNA replication', Journal of Cell Science, vol. 102, no. 3, pp. 393-400.
Seino, Hiroaki ; Hisamoto, Naoki ; Uzawa, Satoru ; Sekiguchi, Takeshi ; Nishimoto, Takeharu. / DNA-binding domain of RCC1 protein is not essential for coupling mitosis with DNA replication. In: Journal of Cell Science. 1992 ; Vol. 102, No. 3. pp. 393-400.
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