DNA conformation-dependent activities of human mitochondrial RNA polymerase

Atsushi Fukuoh, Kippei Ohgaki, Hinako Hatae, Isao Kuraoka, Yoshimasa Aoki, Takeshi Uchiumi, Howard T. Jacobs, Dongchon Kang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Mitochondrial RNA polymerase (POLRMT) is a core protein for mitochondrial DNA (mtDNA) transcription. In addition, POLRMT is assumed to be involved in replication, although its exact role is not yet clearly elucidated. We have found novel properties of human POLRMT using a reconstituted transcription system. Various lengths of RNA molecules were synthesized from templates even without a defined promoter sequence, when we used supercoiled circular double-stranded DNA as a template. This promoter-independent activity was as strong as the promoter-dependent one. Promoter-independent DNA conformation-dependent transcription required TFB2M. On supercoiled templates, the promoter-independent activity was strongly suppressed by a putatively physiological amount of TFAM, while promoter-dependent transcription was inhibited to a lesser extent. These different inhibition patterns by TFAM may be important for prevention of random RNA synthesis in vivo. Promoter-independent activity was also observed on relaxed circular single-stranded DNA, where its activity no longer required TFB2M. RNA synthesis on single-stranded DNA was weakly suppressed by a putatively physiological amount of TFAM but restored by the addition of mitochondrial single-stranded DNA binding protein. We suggest that these properties of POLRMT could explain the characteristic features of mammalian mtDNA transcription and replication.

Original languageEnglish
Pages (from-to)1029-1042
Number of pages14
JournalGenes to Cells
Volume14
Issue number8
DOIs
Publication statusPublished - Aug 6 2009

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Nucleic Acid Conformation
DNA-Directed RNA Polymerases
Mitochondrial DNA
Human Activities
Single-Stranded DNA
RNA
Circular DNA
DNA-Binding Proteins
DNA Replication
DNA
mitochondrial RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this

DNA conformation-dependent activities of human mitochondrial RNA polymerase. / Fukuoh, Atsushi; Ohgaki, Kippei; Hatae, Hinako; Kuraoka, Isao; Aoki, Yoshimasa; Uchiumi, Takeshi; Jacobs, Howard T.; Kang, Dongchon.

In: Genes to Cells, Vol. 14, No. 8, 06.08.2009, p. 1029-1042.

Research output: Contribution to journalArticle

Fukuoh, A, Ohgaki, K, Hatae, H, Kuraoka, I, Aoki, Y, Uchiumi, T, Jacobs, HT & Kang, D 2009, 'DNA conformation-dependent activities of human mitochondrial RNA polymerase', Genes to Cells, vol. 14, no. 8, pp. 1029-1042. https://doi.org/10.1111/j.1365-2443.2009.01328.x
Fukuoh, Atsushi ; Ohgaki, Kippei ; Hatae, Hinako ; Kuraoka, Isao ; Aoki, Yoshimasa ; Uchiumi, Takeshi ; Jacobs, Howard T. ; Kang, Dongchon. / DNA conformation-dependent activities of human mitochondrial RNA polymerase. In: Genes to Cells. 2009 ; Vol. 14, No. 8. pp. 1029-1042.
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