DNA-Damaging Activity and Mutagenicity of Red-Pigment Derived from Ascorbic Acid

Michiko Nonaka, Masata Mitsuiki, Tetsushi Kodama, Hirohisa Omura

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Red-pigment [2,2-nitrilodi-2(2)-deoxy-l-ascorbic acid monoammonium salt (NDA)] was synthesized by the reaction of dehydroascorbic acid with l-phenylalanine. The DNA-damaging activity and the mutagenicity of NDA were assayed by means of DNA-repair and two different gene mutation assays using bacteria. NDA was positive in the spore rec-assay system using Bacillus subtilis without S9 mix, showing that its DNA-damaging index was relatively weak. NDA was positive in both the Ames test and the induced mutation frequency (IMF) assay using Salmonella typhimurium strain TA100 or TA98 with or without S9 mix, revealing that the mutagenic potential was relatively weak. These results suggested that further in vivo tests should be carried out to predict whether NDA might be a carcinogenic or genetic hazard to man.

Original languageEnglish
Number of pages1
JournalFood Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
Volume32
Issue number1
DOIs
Publication statusPublished - Jan 1 1991

Fingerprint

mutagenicity
Ascorbic Acid
ascorbic acid
pigments
Dehydroascorbic Acid
DNA
assays
Mutation Rate
Salmonella typhimurium
Bacillus subtilis
Spores
Phenylalanine
DNA Repair
dehydroascorbic acid
Ames test
Salts
DNA repair
Bacteria
Salmonella Typhimurium
mutagenesis

All Science Journal Classification (ASJC) codes

  • Food Science
  • Public Health, Environmental and Occupational Health

Cite this

DNA-Damaging Activity and Mutagenicity of Red-Pigment Derived from Ascorbic Acid. / Nonaka, Michiko; Mitsuiki, Masata; Kodama, Tetsushi; Omura, Hirohisa.

In: Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi), Vol. 32, No. 1, 01.01.1991.

Research output: Contribution to journalArticle

Nonaka, Michiko ; Mitsuiki, Masata ; Kodama, Tetsushi ; Omura, Hirohisa. / DNA-Damaging Activity and Mutagenicity of Red-Pigment Derived from Ascorbic Acid. In: Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi). 1991 ; Vol. 32, No. 1.
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