DNA detection method based on the microbead velocity under traveling wave dielectrophoresis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Polymerase chain reaction (PCR) is a highly specific and sensitive detection method for bacterial and viral infections by amplifying the specific regions of DNA or RNA via enzymatic reaction. The authors have developed a rapid DNA detection method based on the dielectrophoresis (DEP) characteristic of DNA labeled microbeads for the rapid detection of the DNA that amplified by PCR. This method is based on the change of the Clausius-Mossotti (CM) factor K of DEP when DNA is attached onto microbeads. In former studies, we developed a new DNA detection method based on the change of the real part of K (Re[K]). However, this method requires a large amount of DNA attaching to a microbead to alter the microbead DEP behaviour for DNA detection. In this study, we focus on the imaginary part of K (Im[K]), which theoretically will change more dramatically than Re[K] when DNA is attached onto the microbeads. Since the traveling wave dielectrophoresis behaviour is based on the Im[K], we propose a new method for DNA detection based on the traveling wave dielectrophoresis (twDEP) of microbeads.

Original languageEnglish
Title of host publicationBIODEVICES 2019 - 12th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019
EditorsAna Roque, Ana Fred, Hugo Gamboa
PublisherSciTePress
Pages21-25
Number of pages5
ISBN (Electronic)9789897583537
Publication statusPublished - Jan 1 2019
Event12th International Conference on Biomedical Electronics and Devices, BIODEVICES 2019 - Part of 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019 - Prague, Czech Republic
Duration: Feb 22 2019Feb 24 2019

Publication series

NameBIODEVICES 2019 - 12th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019

Conference

Conference12th International Conference on Biomedical Electronics and Devices, BIODEVICES 2019 - Part of 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019
CountryCzech Republic
CityPrague
Period2/22/192/24/19

Fingerprint

Electrophoresis
DNA
Polymerase chain reaction
RNA

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Electrical and Electronic Engineering

Cite this

Ding, Z., Nakano, M., & Suehiro, J. (2019). DNA detection method based on the microbead velocity under traveling wave dielectrophoresis. In A. Roque, A. Fred, & H. Gamboa (Eds.), BIODEVICES 2019 - 12th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019 (pp. 21-25). (BIODEVICES 2019 - 12th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019). SciTePress.

DNA detection method based on the microbead velocity under traveling wave dielectrophoresis. / Ding, Zhenhao; Nakano, Michihiko; Suehiro, Junya.

BIODEVICES 2019 - 12th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019. ed. / Ana Roque; Ana Fred; Hugo Gamboa. SciTePress, 2019. p. 21-25 (BIODEVICES 2019 - 12th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ding, Z, Nakano, M & Suehiro, J 2019, DNA detection method based on the microbead velocity under traveling wave dielectrophoresis. in A Roque, A Fred & H Gamboa (eds), BIODEVICES 2019 - 12th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019. BIODEVICES 2019 - 12th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019, SciTePress, pp. 21-25, 12th International Conference on Biomedical Electronics and Devices, BIODEVICES 2019 - Part of 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019, Prague, Czech Republic, 2/22/19.
Ding Z, Nakano M, Suehiro J. DNA detection method based on the microbead velocity under traveling wave dielectrophoresis. In Roque A, Fred A, Gamboa H, editors, BIODEVICES 2019 - 12th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019. SciTePress. 2019. p. 21-25. (BIODEVICES 2019 - 12th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019).
Ding, Zhenhao ; Nakano, Michihiko ; Suehiro, Junya. / DNA detection method based on the microbead velocity under traveling wave dielectrophoresis. BIODEVICES 2019 - 12th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019. editor / Ana Roque ; Ana Fred ; Hugo Gamboa. SciTePress, 2019. pp. 21-25 (BIODEVICES 2019 - 12th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019).
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