Abstract
Polymerase chain reaction (PCR) is a highly specific and sensitive detection method for bacterial and viral infections by amplifying the specific regions of DNA or RNA via enzymatic reaction. The authors have developed a rapid DNA detection method based on the dielectrophoresis (DEP) characteristic of DNA labeled microbeads for the rapid detection of the DNA that amplified by PCR. This method is based on the change of the Clausius-Mossotti (CM) factor K of DEP when DNA is attached onto microbeads. In former studies, we developed a new DNA detection method based on the change of the real part of K (Re[K]). However, this method requires a large amount of DNA attaching to a microbead to alter the microbead DEP behaviour for DNA detection. In this study, we focus on the imaginary part of K (Im[K]), which theoretically will change more dramatically than Re[K] when DNA is attached onto the microbeads. Since the traveling wave dielectrophoresis behaviour is based on the Im[K], we propose a new method for DNA detection based on the traveling wave dielectrophoresis (twDEP) of microbeads.
| Original language | English |
|---|---|
| Title of host publication | BIODEVICES 2019 - 12th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019 |
| Editors | Ana Roque, Ana Fred, Hugo Gamboa |
| Publisher | SciTePress |
| Pages | 21-25 |
| Number of pages | 5 |
| ISBN (Electronic) | 9789897583537 |
| Publication status | Published - Jan 1 2019 |
| Event | 12th International Conference on Biomedical Electronics and Devices, BIODEVICES 2019 - Part of 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019 - Prague, Czech Republic Duration: Feb 22 2019 → Feb 24 2019 |
Publication series
| Name | BIODEVICES 2019 - 12th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019 |
|---|
Conference
| Conference | 12th International Conference on Biomedical Electronics and Devices, BIODEVICES 2019 - Part of 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019 |
|---|---|
| Country | Czech Republic |
| City | Prague |
| Period | 2/22/19 → 2/24/19 |
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All Science Journal Classification (ASJC) codes
- Biomedical Engineering
- Electrical and Electronic Engineering
Cite this
DNA detection method based on the microbead velocity under traveling wave dielectrophoresis. / Ding, Zhenhao; Nakano, Michihiko; Suehiro, Junya.
BIODEVICES 2019 - 12th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019. ed. / Ana Roque; Ana Fred; Hugo Gamboa. SciTePress, 2019. p. 21-25 (BIODEVICES 2019 - 12th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - DNA detection method based on the microbead velocity under traveling wave dielectrophoresis
AU - Ding, Zhenhao
AU - Nakano, Michihiko
AU - Suehiro, Junya
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Polymerase chain reaction (PCR) is a highly specific and sensitive detection method for bacterial and viral infections by amplifying the specific regions of DNA or RNA via enzymatic reaction. The authors have developed a rapid DNA detection method based on the dielectrophoresis (DEP) characteristic of DNA labeled microbeads for the rapid detection of the DNA that amplified by PCR. This method is based on the change of the Clausius-Mossotti (CM) factor K of DEP when DNA is attached onto microbeads. In former studies, we developed a new DNA detection method based on the change of the real part of K (Re[K]). However, this method requires a large amount of DNA attaching to a microbead to alter the microbead DEP behaviour for DNA detection. In this study, we focus on the imaginary part of K (Im[K]), which theoretically will change more dramatically than Re[K] when DNA is attached onto the microbeads. Since the traveling wave dielectrophoresis behaviour is based on the Im[K], we propose a new method for DNA detection based on the traveling wave dielectrophoresis (twDEP) of microbeads.
AB - Polymerase chain reaction (PCR) is a highly specific and sensitive detection method for bacterial and viral infections by amplifying the specific regions of DNA or RNA via enzymatic reaction. The authors have developed a rapid DNA detection method based on the dielectrophoresis (DEP) characteristic of DNA labeled microbeads for the rapid detection of the DNA that amplified by PCR. This method is based on the change of the Clausius-Mossotti (CM) factor K of DEP when DNA is attached onto microbeads. In former studies, we developed a new DNA detection method based on the change of the real part of K (Re[K]). However, this method requires a large amount of DNA attaching to a microbead to alter the microbead DEP behaviour for DNA detection. In this study, we focus on the imaginary part of K (Im[K]), which theoretically will change more dramatically than Re[K] when DNA is attached onto the microbeads. Since the traveling wave dielectrophoresis behaviour is based on the Im[K], we propose a new method for DNA detection based on the traveling wave dielectrophoresis (twDEP) of microbeads.
UR - http://www.scopus.com/inward/record.url?scp=85064612244&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85064612244&partnerID=8YFLogxK
M3 - Conference contribution
T3 - BIODEVICES 2019 - 12th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019
SP - 21
EP - 25
BT - BIODEVICES 2019 - 12th International Conference on Biomedical Electronics and Devices, Proceedings; Part of 12th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2019
A2 - Roque, Ana
A2 - Fred, Ana
A2 - Gamboa, Hugo
PB - SciTePress
ER -